文献类型：期刊文献

中文题名：红外光谱法对可生物降解聚合物微球中血红蛋白含量的测定

英文题名：Determination of Hemoglobin in Biodegradable Polymer Microspheres by Infrared Spectroscopy

作者：陈利根[1];单晓茜[1];刘昌胜[1]

机构：[1]华东理工大学材料科学与工程学院,上海200237

年份：2008

卷号：27

期号：7

起止页码：776

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市纳米专项基金资助项目(0452nm022)

语种：中文

中文关键词：红外光谱;血红蛋白;生物降解;聚合物;微球

外文关键词：infrared spectroscopy;hemoglobin;biodegradation;polymer;microspheres

摘要：建立了可生物降解聚合物微球中血红蛋白含量的红外光谱分析方法。选择合适的内标物,排除了聚合物壳材和表面活性剂残留的光谱干扰,不需对样品进行分离。工作曲线的相关系数达0.999 8,回收率为98%-100%,相对标准偏差小于4.2%。
A method for the determination of hemoglobin content in biodegradable polymer microspheres by infrared spectroscopy was developed.By using a appropriate absorption peak and internal standard,the spectral and matrix interferences generated from the internal standard,remnant surfactants and polymer shell were eliminated,thus sample separation was not required prior to analysis.The correlation coefficient of the calibration curve was 0.999 8.The recovery range was 98%-100% for six samples and the relative standard deviation（RSD） was less than 4.2%.The method is rapid and simple.