文献类型：期刊文献

英文题名：ABA-responsive transcription factor bZIP1 is involved in modulating biosynthesis of phenolic acids and tanshinones in Salvia miltiorrhiza

作者：Deng, Changping[1,2,3];Shi, Min[1];Fu, Rong[3];Zhang, Yi[3];Wang, Qiang[3];Zhou, Yang[3];Wang, Yao[3];Ma, Xingyuan[2];Kai, Guoyin[1,3]

机构：[1]Zhejiang Chinese Med Univ, Coll Pharm, Lab Med Plant Biotechnol, Hangzhou 310053, Zhejiang, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Normal Univ, Sch Life Sci, Inst Plant Biotechnol, Shanghai 200234, Peoples R China

年份：2020

卷号：71

期号：19

起止页码：5948

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000577075400021)】；

基金：This work was supported by the National Natural Science Fund of China (81522049, 31571735, 31270007, and 30900110), Shanghai Science and Technology Committee Project (17JC1404300 and 15430502700), Zhejiang Provincial Wanren Program for Leading Talents of Science and Technology Innovation (2018R52050), Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents, New Century Talent Project (NECT-13-0902), the `Dawn' Program of Shanghai Education Commission (16SG38), and the Opening project of Zhejiang provincial preponderant and characteristic subject of Key University (Traditional Chinese Pharmacology), Zhejiang Chinese Medical University (ZYAOX2018019; ZYAOXYB2019005)

语种：英文

外文关键词：Biosynthesis; phenolic acids; Salvia miltiorrhiza; tanshinones; transcriptional regulation; transcription factor

摘要：Phenolic acids and tanshinones are major bioactive ingredients in Salvia miltiorrhiza, which possess pharmacological activities with great market demand. However, transcriptional regulation of phenolic acid and tanshinone biosynthesis remains poorly understood. Here, a basic leucine zipper transcription factor (TF) named SmbZIP1 was screened from the abscisic acid (ABA)-induced transcriptome library. Overexpression of SmbZIP1 positively promoted phenolic acid biosynthesis by enhancing expression of biosynthetic genes such as cinnamate-4-hydroxylase (C4H1). Furthermore, biochemical experiments revealed that SmbZIP1 bound the G-Box-like1 element in the promoter of the C4H1 gene. Meanwhile, SmbZIP1 inhibited accumulation of tanshinones mainly by suppressing the expression of biosynthetic genes including geranylgeranyl diphosphate synthase (GGPPS) which was confirmed as a target gene by in vitro and in vivo experiments. In contrast, the phenolic acid content was reduced and tanshinone was enhanced in CRISPR/Cas9 [clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9]-mediated knockout lines. In addition, the previously reported positive regulator of tanshinone biosynthesis, SmERF1L1, was found to be inhibited in SmbZIP1 overexpression lines indicated by RNA sequencing, and was proven to be the target of SmbZIP1. In summary, this work uncovers a novel regulator and deepens our understanding of the transcriptional and regulatory mechanisms of phenolic acid and tanshinone biosynthesis, and also sheds new light on metabolic engineering in S. miltiorrhiza.