文献类型：期刊文献

英文题名：Comparative effects of schisandrin A, B, and C on Propionibacterium acnes-induced, NLRP3 inflammasome activation-mediated IL-1β secretion and pyroptosis

作者：Guo, Miaomiao[1];An, Faliang[1];Yu, Haiyuan[1];Wei, Xing[1];Hong, Minhua[2];Lu, Yanhua[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Inoherb Co Ltd, Technol Ctr, 121 Chengyin Rd, Shanghai 200083, Peoples R China

年份：2017

卷号：96

起止页码：129

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000418502100017)】；

基金：This work was supported by Shanghai Baoshan Government-Industry-University-Institute collaboration (16-C-22), the General Financial Grant from the China Postdoctoral Science Foundation (2016M601532), the National Natural Science Foundation of China (No. 31600273), and Shanghai Inoherb Co. Ltd.

语种：英文

外文关键词：Schisandrin; Anti-inflammatory; Propionibacterium acnes; NLRP3 inflammasome; Pyroptosis; Caspase-1

摘要：Propionibacterium acnes, a common pathogen associated with acne, is also responsible for various surgical infections. Schisandrin A, schisandrin B and schisandrin C, the representative lignans of Schisandra chinensis (Turcz.) Baill. extract, inhibit P. acnes-induced inflammation. However, their effects on P. acnes-induced IL-1 beta secretion and pyroptosis mediated by NLRP3 inflammasome activation remain unknown. In this study, we compared the effects of schisandrin A, B, and C (Sch A, B, and C) on IL-1 beta secretion and pyroptosis in P. acnes-infected THP-1 cells. As NLRP3 plays important roles in P. acnes-mediated inflammation and pyroptosis, we also investigated the effects of Schs on P. acnes-induced NLRP3 inflammasome activation by measuring the levels of NLRP3, active caspase-1, and mature IL-1 beta, and activity of caspase-1. Our results showed that Sch A, B, and C suppressed P. acnes-induced pyroptosis. Further, the three lignans significantly suppressed NLRP3 inflammasome activation, with the following potency: Sch C > Sch B > Sch A. Three lignans also inhibited the production of mitochondrial ROS and ATP release. Additionally, Sch B and C almost completely prevented the efflux of K+, whereas Sch A had a relatively weak effect. Collectively, our novel findings showed that Sch A, B, and C effectively suppressed IL-1 beta secretion and pyroptosis by inhibiting NLRP3 inflammasome activation in P. acnes-infected THP-1 cells. Thus, Schs may be promising agents for the treatment of P. acnes-related infections.