文献类型：期刊文献

英文题名：Simple and Cheap CRISPR/Cas12a Biosensor Based on Plug-and-Play of DNA Aptamers for the Detection of Endocrine-Disrupting Compounds

作者：Wang, Feng-Qing[1,2]; Zhao, Yunqiu[1,2]; Zhu, Lin[1]; Ding, Yaxue[1]; Ji, Weiting[1]; Liu, Kun[1]; Liu, Ke[1,2]; Gao, Bei[1,2]; Tao, Xinyi[1,2]; Dong, Yu-Guo[2]; Wei, Dongzhi[1,2]

机构：[1] State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Key Laboratory of Biocatalysis and Intelligent Manufacturing [ECUST], China National Light Industry, Shanghai, 200237, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230103219)

语种：英文

外文关键词：DNA - Endocrine disrupters - Phenols

摘要：Endocrine-disrupting compounds (EDCs) are widely distributed in the environment. Simple and sensitive detection methods are always required to rapidly assess and prevent their pollution. Here, we present a CRISPR/Cas12a (CAS) biosensor based on DNA aptamers for point-of-care detection of EDCs. To prove the concept, two typical EDCs, 17β-estradiol (E2) and bisphenol A (BPA), were selected to be detected by the CAS biosensors via the plug-and-play of their DNA aptamers. The results indicated that the performance of the CAS biosensors can be well regulated by controlling the trans-cleavage activity of Cas12a on a single-stranded DNA reporter and optimizing the sequence and ratio of DNA aptamer and activator DNA. Ultimately, two reliable and specific biosensors were developed, with the linear range and limit of detection of 0.2-25 nM and 0.08 nM for E2 and of 0.1-250 nM and 0.06 nM for BPA, respectively. Compared to the existing detection methods, the CAS biosensors showed higher reliability and sensitivity with simple operation, short detection time, and no costly equipment, manifesting that it was a practical and efficient tool promisingly in EDCs field detection. ? 2023, The Authors. All rights reserved.