文献类型：期刊文献

英文题名：Horseradish Peroxidase Immobilized on Graphene Oxide: Physical Properties and Applications in Phenolic Compound Removal

作者：Zhang, Feng[2];Zheng, Bin[2];Zhang, Jiali[1];Huang, Xuelei[2];Liu, Hui[2];Guo, Shouwu[1];Zhang, Jingyan[2]

机构：[1]Shanghai Jiao Tong Univ, Natl Key Lab Micronano Fabricat Technol, Key Lab Thin Film & Microfabricat, Minist Educ,Res Inst Micronano Sci & Technol, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：114

期号：18

起止页码：8469

外文期刊名：JOURNAL OF PHYSICAL CHEMISTRY C

收录：;EI(收录号：20101912926432);WOS:【SCI-EXPANDED(收录号:WOS:000277280800048)】；

基金：This work was supported by the State Key Laboratory of Bioreactor Engineering, NSFC of P. R. China (Nos. 20671034 and 20774029), and the National "973 Program" (Nos. 2007CB936000 and 2010CB933900).

语种：英文

外文关键词：Catalyst activity - Enzyme activity - Food products - Phenols - Enzyme immobilization - Physical properties - Substrates - Graphene

摘要：Composition, morphology, and surface characteristics of solid substrates play critical roles in regulating immobilized enzyme activity. Grapheme oxide (GO), a novel nanostructured material, has been illustrated as an ideal enzyme immobilization substrate due to its unique chemical and structural properties. Physical properties and catalytic activity of GO immobilized horseradish peroxidase (HRP) and its application in phenolic compound removal are described in the present study. HRP loading on GO was found to be much higher than that on reported substrates. The GO immobilized HRP showed improved thermal stability and a wide active pH range, attractive for practical applications. The removal of phenolic compounds from aqueous solution using the GO immobilized HRP was explored with seven phenolic compounds as model substrates. The GO immobilized HRP exhibited overall a high removal efficiency to several phenolic compounds in comparison to soluble HRP, especially for 2,4-dimetheoxyphenol and 2-chlorphenol, the latter a major component of industrial wastewater.