文献类型：期刊文献

中文题名：板蓝根多糖的系统分离纯化与组成分析

英文题名：System isolation and purification of Isatidis Radix polysaccharides and determination of their compositions

作者：国欣[1];胡小龙[1];王月荣[1];杨子峰[2];王玉涛[2];李征途[2];胡坪[1]

机构：[1]上海市功能性材料化学重点实验室,华东理工大学化学与分子工程学院,上海200237;[2]广州医科大学附属第一医院,呼吸疾病国家重点实验室,广东广州510230

年份：2016

卷号：47

期号：9

起止页码：1508

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金资助项目(81273481,U1201227,81403167);广州市属高校科研项目(1201430183)

语种：中文

中文关键词：板蓝根多糖;板蓝根糖蛋白;系统分离纯化;单糖组成;氨基酸组成

外文关键词：Isatidis Radix polysaccharides;Isatidis Radix glycoproteins;system isolation and purification;monosaccharide composition;amino acid composition

摘要：目的建立板蓝根多糖的系统分离纯化方法,并对分离得到的均一多糖进行组成测定。方法以抗病毒活性较好的80%醇沉板蓝根粗多糖为研究对象,对其进行DEAE-Sepharose Fast Flow柱色谱、Sephacryl-200葡聚糖凝胶柱色谱以及高效凝胶色谱系统分离纯化。分别采用1-苯基-3-甲基-5-吡唑啉酮(PMP)和邻苯二甲醛(OPA)衍生化HPLC法对分离得到的板蓝根均一多糖进行单糖组成和氨基酸组成测定。结果利用系统分离纯化策略,从80%醇沉板蓝根粗多糖中分离得到2种均一板蓝根多糖(IRPS1A和IRPS1B)和2种均一板蓝根糖蛋白(IRPS2A和IRPS3A)。其中,IRPS1A与IRPS1B的单糖组成均为甘露糖、葡萄糖、半乳糖和阿拉伯糖;IRPS2A的单糖组成为半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖;IRPS3A的单糖组成为甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖。糖蛋白IRPS2A与IRPS3A均含有天门冬氨酸、谷氨酸、丝氨酸、组氨酸、甘氨酸、苏氨酸、精氨酸、丙氨酸、胱氨酸、缬氨酸、苯丙氨酸、异亮氨酸、亮氨酸、赖氨酸,共14种氨基酸残基。同时,IRPS2A还含有酪氨酸残基。结论该系统分离纯化的策略可应用于板蓝根均一多糖(糖蛋白)的获取,为板蓝根多糖的结构分析及药理活性研究奠定基础。
Objective To isolate and purify the polysaccharides(glycoproteins) from Isatidis Radix(Banlangen) systematically and to study the composition of them.Methods Crude polysaccharides precipitated by 80% ethanol from the water extract of Isatidis Radix,which has the anti-viral activity,were fractionated by DEAE-Sepharose Fast Flow,Sephacryl-200,and high gel chromatography system sequentially.The composition of monosaccharides and amino acids of polysaccharides(glycoproteins) was then determined by HPLC with pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone(PMP) and o-phthalaldehyde(OPA),respectively.Results Two of homogeneous polysaccharides named IRPS1 A and IRPS1 B and two of homogeneous glycoproteins named IRPS2 A and IRPS3 A were obtained from Isatidis Radix by systematical separation and purification.The monosaccharide composition of IRPS1 A and IRPS1 B was of arabinose,mannose,galactose,and glucose.IRPS2 A contained galacturonic acid,glucose,galactose,and arabinose.While IRPS3 A contained mannose,rhamnose,galacturonic acid,glucose,galactose,and arabinose.The amino acid compositions of IRPS2 A and IRPS3 A were 14 kinds of amino acid residues including Asp,Glu,Ser,His,Gly,Thr,Arg,Ala,Cys,Val,Phe,Iso,Leu,and Lys.Besides all,IRPS2 A also contained Tyr.Conclusion This strategy can be used for the isolation and purification of homogeneous polysaccharides/glycoproteins from Isatidis Radix which provides a possible support for the elucidation of the structure and the pharmacologic action of Isatidis Radix polysaccharides(glycoproteins).