文献类型：期刊文献

英文题名：Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30

作者：Zhang, Liaoyuan[1];Yang, Yunlong[1];Sun, Jian'an[1];Shen, Yaling[1];Wei, Dongzhi[1];Zhu, Jiawen[2];Chu, Ju

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Chem Engn Res Ctr, Shanghai 200237, Peoples R China

年份：2010

卷号：101

期号：6

起止页码：1961

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000274271400059)】；

基金：This work was supported by Chinese National Program for High Technology and Development (863 Program) (No. 2006AA02Z243), Shanghai Leading Academic Discipline Project (Project: 13505) and State key laboratory special fund (No. 2060204).

语种：英文

外文关键词：2,3-Butanediol; Serratia marcescens H30; Respiratory quotient; Medium optimization; Fed-batch

摘要：The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett-Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by S. marcescens H30 in a 3.71 bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC + BD) productivity of 3.49 g/l h and the yield of 94.67% was obtained. (C) 2009 Elsevier Ltd. All rights reserved.