文献类型：期刊文献

中文题名：应用生物反应器和微载体培养Vero细胞生产猪流行性腹泻病毒的研究

英文题名：Experimental Investigation of PEDV Production Through Vero Cells Cultured on Microcarriers in a Bioreactor System

作者：王建超[1];周燕[1];华平[1];邹勇[2];谭文松[1];钱永清[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海市农业科学院畜牧兽医研究所,201106

年份：2003

卷号：34

期号：6

起止页码：573

中文期刊名：畜牧兽医学报

外文期刊名：ACTA VETERINARIA ET ZOOTECHNICA SINICA

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：上海市科技兴农重点攻关项目〔农科攻(99)第5-1号和农科攻(2001)第3-8号〕

语种：中文

中文关键词：生物反应器;微载体;Vero细胞;培养;猪流行性腹泻病毒;PEDV;生长;代谢;增殖;接种密度;疫苗

外文关键词：Vero cells;PEDV;Microcarrier;Bioreactor

摘要：对应用生物反应器系统和微载体培养Vero细胞生产PEDV进行了实验研究,通过分析和比较批培养及换液培养过程中细胞生长、代谢和病毒增殖的相互关系,发现PEDV随着细胞的生长而不断在胞内增殖和积累,72h后Vero细胞需经过一个短暂的停止生长阶段,再继续呈指数生长。反应器换液培养的单位培养基细胞产量比批培养提高了28%,比方瓶培养提高了138%,疫苗生产效率显著提高。当接种密度为1 23×105cells/ml时,换液培养至96h时获得了1 74×106cells/ml的高细胞密度,细胞扩增了14 3倍,且单位细胞的病毒产量并不低于静态培养。
The potential of Porcine Epidemic Diarrhea Virus(PEDV)production through Vero cells cultured on microcarriers in a bioreactor system was investigated in this work.By analyzing and comparing the relationship between cell growth,metabolism,and virus propagation in the batch and mediumexchange mode,it was found that the virus propagated and accumulated with the cell growth,and the Vero cells existed a temporary growthstop stage about 12h during the exponential growth phase.Cell yield per ml medium consumed in the mediumexchange culture was higher 28% than that in the batch culture mode,and higher 138% than that in flask cultures.In the mediumexchange culture the maximum cell density reached 174×106 cells/ml at the 4th day after seeding,in which the cells was expanded 143 folds.In addition,it was found that virus productivity per cell in a bioreactor system was comparable to that in static flask cultures.