文献类型：期刊文献

英文题名：Spinosad induces autophagy of Spodoptera frugiperda Sf9 cells and the activation of AMPK/mTOR signaling pathway

作者：Yang, Mingjun[1];Hao, Youwu[1];Gao, Jufang[2];Zhang, Yang[1];Xu, Wenping[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life & Environm Sci, 100 Guilin Rd, Shanghai 200234, Peoples R China

年份：2017

卷号：195

起止页码：52

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000398875100006)】；

基金：This work was financially supported by the National Key Technology Research Development Program of China (No. 2011BAE06B04).

语种：英文

外文关键词：Spinosad; Sf9 cells; Autophagy; AMPK; mTOR

摘要：Spinosad, a high-selectivity neural toxin, has been widely used in agricultural production. However, the mode of action of spinosad on insect non-neural cells is not yet clear and hence requires further investigation. Therefore, to reveal the cytotoxic mechanisms of spinosad, we investigated whether and how it can induce autophagic cell death. After treating SIP cells with spinosad, the resulting autophagosome was observed by transmission electron microscopy and monodansylcadaverine staining. Interestingly, spinosad induced the accumulation of Beclin-1, degradation of p62, and intensification of LC3-B formation and translocation and thus autophagy, whereas, 3-MA treatment reverted the phenotype. Under ATP depletion conditions, spinosad induced autophagy of Sf9 cells and activation of the AMPK/mTOR signaling pathway. (C) 2017 Elsevier Inc. All rights reserved.