文献类型：期刊文献

英文题名：A facile fluorescent probe with a large Stokes shift for sequentially detecting copper and sulfide in 100% aqueous solution and imaging them in living cells

作者：Wang, Huan[1];Shi, Dong-Lei[1];Li, Jin[1];Tang, Hao-Yang[2];Li, Jian[3];Guo, Yuan[1]

机构：[1]Northwest Univ, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Coll Chem & Mat Sci, Xian 710127, Shaanxi, Peoples R China;[2]Xian Univ Posts & Telecommun, Sch Automat, Xian 710121, Shaanxi, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2018

卷号：256

起止页码：600

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20174504364927);WOS:【SCI-EXPANDED(收录号:WOS:000414971100071)】；

基金：This work was supported by the National Natural Scientific Foundation of China (No. 21472148 and 21072158), Overseas Students Science and Technology Activities Project Merit Funding of Shaanxi Province (No. 20151190), the Special Foundation of the Education Committee of Shaanxi Province (No. 15JK1674) and Academic Backbone of Northwest University Outstanding Youth Support Program.

语种：英文

外文关键词：Copper ions; Hydrogen sulfide; Fluorescent imaging; Large Stokes shift; Pure water system

摘要：The selective detection of Cu2+ and S2- in pure water systems is very challenging. To address this problem, we here present a novel fluorescent probe CF536 for the reversible detection of Cu2+ and S2- in a 100% aqueous solution via a displacement approach based on fluorescence quenching and the ESIPT mechanism, and characterized by IR, NMR, HRMS and X-ray analysis. The introduction of Cu2+ (1.0 equiv.) to the aqueous solution of CF536 led to quenching of the intense green fluorescence within 2 min. On the addition of S2- (3.0 equiv.), the emission of the ESIPT probe soon recovered from the CF536-Cu2+ system due to the abolishment of paramagnetic Cu2+. The fluorescence "ON-OFF-ON" cycle can be repeated a minimum of 5 times by the alternate addition of Cu2+ and S2-, implying that CF536 is a renewable dual-functional probe. The probe displayed high selectivity, a rapid response time, excellent water solubility due to introduction of the extremely hydrophilic trihydroxyl group and outstanding anti-interference ability for its large Stokes shift of 101 nm (lambda(abs) = 435 nm, lambda(em) = 536 nm), thus making the method highly suitable for bioimaging. The probe CF536 was successfully used for fluorescent imaging of copper and hydrogen sulfide in living cells. (C) 2017 Elsevier B.V. All rights reserved.