文献类型：期刊文献

英文题名：Porosity and Semipermeability of Hemoglobin-Loaded Polymeric Nanoparticles as Potential Blood Substitutes

作者：Sheng, Yan[1,2,3];Liu, Changsheng[1,2,3];Yuan, Yuan[1,2,3];Zhang, Xiaolan[1,2,3];Shan, Xiaoqian[1,2,3];Xu, Feng[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2009

卷号：91B

期号：2

起止页码：631

外文期刊名：JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

收录：;EI(收录号：20095112554515);WOS:【SCI-EXPANDED(收录号:WOS:000270868600018)】；

基金：Contract grant sponsor: National High Technology Research and Development Program of China (863 program); Contract grant number: 2004AA-302050Contract grant sponsor: Shanghai Nanotechnology Special Foundation; Contract grant number: 0452nm022.

语种：英文

外文关键词：porosity; semipermeability; nanoparticles; molecular weight cut-off; probes

摘要：Porosity and semipermeability, allowing life-sustaining small molecules to penetrate, but hemoglobin (Hb) and other enzymes to cut off, predominantly affect the functionalities of the Hb-loaded polymeric nanoparticles (HbPNPs) as blood substitutes. In this article, HbPNPs formulated in the size range of 110-122 nm were prepared by a modified double-emulsion method with poly(lactic acid) (PLA)-based polymers. The influences of the main preparation conditions, including solvent composition, stirring speed, Hb concentration and polymer matrix, on the porosity were investigated in details. To evaluate the porosity of HbPNPs, a novel nondestructive testing method based on molecular weight cut-off (MWCO) was developed, and an effusion approach was applied to investigate the pore size in the particle shells with poly(ethylene glycol)s (PEGs) of different molecular weights (PEG200, PEG400, PEG600) as probes. Moreover, in vitro diffusion behaviors of ascorbic acid and reduced glutathione from HbPNPs fabricated with various polymer matrices were studied. The MWCO of HbPNPs by changing solvent composition, stirring speed, Hb concentration, and polymer composition varied from 200 to 600, especially the PEGylation of the polymer, which exhibited obvious influence on the MWCO of HbPNPs. Ascorbic acid with molecular weight 176.1 could diffuse into PEGylated nanoparticles with mPEG content of 5-30 wt % freely, while reduced glutathione with molecular weight 307.3 could not penetrate when mPEG content reached 30 wt %. These results suggest that the HbPNPs optimized with MWCO between 400 and 600 can facilitate the transport of all those life-sustaining small molecules. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 91B: 631-642, 2009