文献类型：期刊文献

中文题名：基本培养基中芳香族氨基酸和维生素对D-核糖生产的影响

英文题名：Effect of Aromatic Amino Acids and Vitamins on D-Ribose Production and Optimization of Minimal Medium

作者：吴琳[1];李志敏[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2009

卷号：35

期号：5

起止页码：707

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：枯草芽孢杆菌;D-核糖;烟酸;吡哆醛

外文关键词：Bacillus subtilis; D-ribose;nicotinic acid; pyridoxine

摘要：考察了芳香族氨基酸和维生素对转酮醇酶缺失的枯草芽孢杆菌生长以及D-核糖生产的影响。实验结果表明:L-酪氨酸、L-色氨酸、L-苯丙氨酸、生物素、烟酸、吡哆醛的添加能改善菌体生长,提高D-核糖产量,特别是烟酸作用尤其明显。在单因素实验基础上,采用响应面分析方法对培养基中的维生素添加量进行了优化,确定最佳培养基组成。在此优化培养基中D-核糖质量浓度达到(23.4±0.39)g/L,与预测结果一致,是不添加维生素时核糖产量的2.31倍,D-核糖对葡萄糖的得率达到0.353 mol/mol。
In this study, effects of aromatic amino acids and vitamins on the growth of transketolasedeficient Bacillus subtilis and D-ribose biosynthesis were studied in chemically defined media. It was found that aromatic amino acids, biotin, pyridoxine and nicotinic acid promoted cell growth, and nicotinic acid enhanced D-ribose production significantly. The response surface methodology （RSM） was applied to optimize the concentration of vitamins in the minimal medium. Under optimal condition, the production of D-ribose was enhanced to （23.4±0.39） g/L, which is about 2.31-fold compared with the case without vitamins supplementation, and the molar yield based on glucose reached 0. 353 mol/mol.