文献类型：期刊文献

英文题名：A putative redox-sensing regulator Rex regulates lincomycin biosynthesis in Streptomyces lincolnensis

作者：Hou, Bingbing[1,2];Wang, Ruida[1,2];Zou, Jingyun[1,2];Zhang, Feixue[1,2];Wu, Haizhen[1,2];Ye, Jiang[1,2];Zhang, Huizhan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai, Peoples R China

年份：2021

卷号：61

期号：9

起止页码：772

外文期刊名：JOURNAL OF BASIC MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000677721300001)】；

基金：China Postdoctoral Science Foundation, Grant/Award Number: 2019M650079; National Natural Science Foundation of China, Grant/Award Number: 31900059

语种：英文

外文关键词：Streptomyces lincolnensis; lincomycin; Rex; transcriptional regulation

摘要：Lincomycin is an important antimicrobial agent which is widely used in clinical and animal husbandry. The biosynthetic pathway of lincomycin comes to light in the past 10 years, however, the regulatory mechanism is still unclear. In this study, a redox-sensing regulator Rex from Streptomyces lincolnensis (Rex(lin)) was identified and characterized to affect cell growth and lincomycin biosynthesis. Disruption of rex resulted in an increase in cell growth, but a decrease in lincomycin production. The results of quantitative real-time polymerase chain reaction showed that Rex(lin) can promote transcription of the regulatory gene lmbU and the structural genes lmbA, lmbC, lmbJ, lmbV, and lmbW. However, electrophoretic mobility shift assay analysis demonstrated that Rex(lin) can not bind to the promoter regions of these genes above. Findings in this study broadened our horizons in the regulatory mechanism of lincomycin production and laid a foundation for strain improvement of antibiotic producers.