文献类型：期刊文献

英文题名：HPLC determination of massoia lactone in fermentedCordyceps sinensismycelium Cs-4 and its anticancer activity in vitro

作者：Sang, Qingni[1];Pan, Yu[2];Jiang, Zhihong[2];Wang, Yuerong[1];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Taipa 999078, Macau, Peoples R China

年份：2020

卷号：44

期号：9

外文期刊名：JOURNAL OF FOOD BIOCHEMISTRY

收录：;EI(收录号：20203008981849);WOS:【SCI-EXPANDED(收录号:WOS:000552204300001)】；

基金：National Natural Science Foundation of China, Grant/Award Number: 81973285

语种：英文

外文关键词：Cordyceps sinensis; cytotoxicity; HPLC determination; massoia lactone; taxol-resistant cell line; volatile oil

摘要：The fermentation product ofCordyceps sinensismycelium Cs-4 was commonly used as alternative substitutes of naturalC. sinensis. Massoia lactone is the dominant component in the volatile oil of Cs-4 mycelium. In this research, we present a high performance liquid chromatography (HPLC) method for the quantitation of massoia lactone in Cs-4 mycelium. The high and stable contents of massoia lactone with values of 2.98-3.77 mg/g, indicated that massoia lactone could be considered as a marker for the quality assessment of this product. The results of MTT and CCK-8 assay showed that Cs-4 mycelium volatiles exhibited cytotoxicity against eight malignant tumor cells (IC50 = 6.0-49.8 mu g/ml) in comparison to the anticancer drug 5-fluorouracil (IC50 = 17.0-425.3 mu g/ml), and massoia lactone might be the chemical basis for the anticancer effects of Cs-4 mycelium. Compared to the commercial drugs paclitaxel and docetaxel (IC50 = 253-1973 mu g/ml), the Cs-4 mycelium volatiles and massoia lactone were discovered to possess inhibitory to taxol-resistant cell lines (IC50 = 1.5-8.6 mu g/ml). Practical applications Considering that there is still a lack of marker components distinctive to Cs-4 mycelium, the HPLC method represents a useful tool for the quality evaluation of Cs-4 mycelium. Moreover, the volatile oil of Cs-4 mycelium and massoia lactone have prominent anticancer property in vitro. It gives a clue that Cs-4 mycelium, the volatile oil and massoia lactone could be potentially employed in the food and medical industries for its anticancer applications.