文献类型：期刊文献

英文题名：Functional suppression of macrophages derived from THP-1 cells by environmentally-relevant concentrations of arsenite

作者：Xu, Huan[1,2];Wang, Xiaolei[1];Wang, Wei[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut Sci, Rm 403,Lab Bldg 14 West,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Univ New Mexico, Dept Chem & Chem Biol, Albuquerque, NM 87131 USA

年份：2018

卷号：214

起止页码：36

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000447569500006)】；

基金：This work was funded by National Natural Science Foundation of China (grant No. 21738002). This work was also funded by a research grant from East China University of Science and Technology (grant No. YC 0142125).

语种：英文

外文关键词：Arsenite; Macrophage; THP-1 cells; Environmentally-relevant concentrations; Phagocytosis; Cytokine production; Nitric oxide

摘要：Environmental exposure to arsenic is known to induce immunotoxicity. Macrophages are the professional phagocytes that are important in the immune system. In this study, we utilized the macrophages derived from the THP-1 human monocyte cell line as the experimental model to study the functional suppression induced by arsenite (As+3), one of the most prevalent forms of inorganic arsenic, at environmentally-relevant concentrations. Apoptosis was observed in the THP-1 derived macrophages treated with 500 nM As+3 for 18 h. Suppression of phagocytosis was induced by 18 h As+3 treatment starting from 100 nM. Suppressive effects on the production of two pro-inflammatory cytokines, IL-1 beta and TNF-alpha, were also found with the treatment of low to moderate doses of As+3 in lipopolysaccharides-stimulated THP-1 derived macrophages. The nitric oxide production was also inhibited by As+3 treatments, which was negatively correlated with the production of superoxide. Collectively, the results from the study demonstrated that environmentally-relevant concentrations of As+3 induced cytotoxicity and suppressed the major cellular functions in THP-1 derived macrophages. The macrophages were showed to be relatively sensitive to As+3, and could be the essential target of the toxicity induced by environmental arsenic exposures.