文献类型：期刊文献

中文题名：妥布霉素发酵中的二次生长

英文题名：The second growth phenomenon of S.tenebrarius in tobramycin fermentation

作者：许学书[1];方夏虹[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：26

期号：1

起止页码：32

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：生物反应器国家重点实验室资助

语种：中文

中文关键词：妥布霉素;黑暗链霉菌;供氧条件;次级代谢

外文关键词：Tobramycin; S.tenbrarius ; Oxygen supply; Secondarg metabolism

摘要：黑暗链霉菌产生次级代谢产物妥布霉素的过程与供氧条件密切相关。通过对常规发酵的溶氧和残糖变化规律研究发现发酵后期有菌体的二次生长现象 ,影响了妥布霉素的分泌。采用在发酵后期降低转速的方法抑制发酵过程中黑暗链霉菌的二次生长 ,延长了妥布霉素的分泌期 ,使最终效价比常规发酵提高了2 7%。
The procedure during which the second metabolic product tobramycin is secreted closely depends on the condition of oxyegen supply. The phenomenal of second time growth of S.tenebrarius during the procedure was found by studying the lure of DO and residual sugar change in the common fermentation, which affected on the secretion of tobramycin. The second time growth of S.tenebrarius was inhibited and the secretion period was extended by decreasing the agitation rate at the later stage of the fermentation. It resulted in the increase of final tobramycin concentration which was 27% more than those in average fermentation batches.