文献类型：期刊文献

中文题名：β-环糊精对SOD的化学修饰

英文题名：STUDY ON PHYSICOCHEMICAL PROPERTIES OF Cu,ZnSUPEROXIDE DISMUTASE MODIFIED WITH βCYCLODEXTRIN

作者：陈吉祥[1,2];袁勤生[1,2]

机构：[1]华东理工大学生物工程学院;[2]中国农业科学院中兽医研究所

年份：1998

卷号：29

期号：2

起止页码：59

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：铜锌超氧化物歧化酶;β-环糊精;化学修饰;理化性质

外文关键词：superoxide dismutase, βcyclodextrin, modification, physicochemical properties

摘要：牛血铜锌－超氧化物歧化酶（Ｃｕ，Ｚｎ－ＳＯＤ）用β－环糊精修饰后，其理化性质发生了部分变化：修饰后的酶呈不均一性，在聚丙烯酰胺凝胶电泳出现４条带，在薄层平板等电聚焦电泳中，等电点降低，修饰酶的紫外吸收光谱未见明显改变，抗蛋白酶水解能力增加。
Bovine Cu, Znsuperoxide dismutase was modified with βcyclodextrin(βCD) activated by NaIO4. As compared with that of native SOD, the physicochemical properties of the modified enzyme changed partly. The modified SOD was highly heterogeneous with at least 4 bands on the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE), and showed lower isoelectric point on the thin layer isoelectric focusing but no obvious change on the ultraviolet spectra. The modified SOD was more resistent to enzymic degradation.