文献类型：期刊文献

中文题名：高对映选择性环氧化物水解酶产生菌的筛选及特性研究

英文题名：ISOLATION AND CHARACTERIZATION OF AN EPOXIDE HYDROLASE PRODUCER FOR ENANTIOSELEC TIVE HYDROLYSIS OF (R,S)-PHENYL GLYCIDYL ETHER

作者：唐燕发[1];许建和[1];武慧渊[1];叶勤[1];Schulze Birgit[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室生物催化研究室,上海200237;[2]DSM Research,P.O.Box18

年份：2001

卷号：28

期号：5

起止页码：14

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：荷兰DSM公司资助项目 (No.ACER 980 8 0 1 2 6)

语种：中文

中文关键词：缩水甘油苯基醚;手性环氧化物;巨大芽孢杆菌;环氧化物水解酶;菌株筛选;对映选择性;水解

外文关键词：Phenyl glycidyl ether,Chiral epoxide,Bacillus megaterium,Epoxide hydrolase,Enantioselective hydrolysis

摘要：从土壤中分离的芽孢杆菌BacillusmegateriumECU1 0 0 1所产环氧化物水解酶能高对映选择性水解缩水甘油苯基醚 (对映选择率E值可达 47 8) ,当转化率为 5 5 9%时 ,剩余的 (S) 缩水甘油苯基醚的光学纯度 (对映体过量值 ,ee)可达 99 5 % ;当底物浓度提高到 60mmol/L时 ,光学纯 (S) 缩水甘油苯基醚的收率达到 2 5 6%
A bacterial strain (ECU1001) was isolated from soil and identified to be Bacillus megaterium.The strain shows an activity of epoxide hydrolase that catalyzes the asymmetric hydrolysis of phenyl glycidyl ether,yielding (S)-epoxide and (R)-diol with high enantioselectivity (E=47.8).When the racemic substrate was enzymaticlly hydrolyzed to 55.9% conversion,the enantiomeric excess (ee) of recovered (S)-epoxide was 99.5%.The yield of (S)-phenyl glycidyl ether could reach 25.6% when concentration of the substrate was increased to 60 mmol/L.