文献类型：期刊文献

中文题名：Mechanisms of antimicrobial peptides as characterized by solid-state NMR

作者：Rong Han[1,2];Shenlin Wang[1,2,3]

机构：[1]College of Chemistry and Molecular Engineering and Beijing NMR Center,Peking University,Beijing,100871,China;[2]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China;[3]Beijing National Laboratory for Molecular Science,Beijing,100871,China

年份：2022

卷号：2

期号：2

起止页码：119

中文期刊名：Magnetic Resonance Letters

外文期刊名：磁共振快报（英文）

基金：supported by the National Key Research and Development Program of the Ministry of Science and Technology,People's Republic of China(contract number 2016YFA0501203);the National Natural Science Foundation of China(21874004,31470727);the Interdisciplinary Medicine Seed Fund of Peking University,and the Fundamental Research Funds for the Central University.

语种：英文

中文关键词：Antimicrobial peptides(AMP);Solid-state NMR(SSNMR);Sample preparation;Antimicrobial mechanisms;Whole-cell NMR

摘要：Antimicrobial peptides(AMP)are small proteins that play critical roles in host defense against microbe invasion.Many AMPs disrupt the cellular membrane of microbe,while the mechanism of action of AMPs can be very sophisticated.Solid-state NMR(SSNMR)technique is powerful in characterizing the mechanism of AMPs in vivo and in vitro.This review summarizes the recent advance of SSNMR technique in AMP mechanisms characterization.We highlight the sample preparation approaches,the SSNMR spectroscopic methods,and a number of outstanding examples of AMP mechanisms elucidated via SSNMR spectroscopy.