文献类型：期刊文献

英文题名：Efficacy of Montanide? ISA 763 A VG as aquatic adjuvant administrated with an inactivated Vibrio harveyi vaccine in turbot (Scophthalmus maximus L.)

作者：Xu, Wenting[1,2];Jiao, Chenglong[1,2];Bao, Pengcheng[1,2];Liu, Qin[1,2];Wang, Pengbo[3];Zhang, Ruilin[4];Liu, Xiaohong[1,2];Zhang, Yuanxing[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Shanghai Wei Sheng Marine Biotechnol Co Ltd, Shanghai 200237, Peoples R China;[4]Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China

年份：2019

卷号：84

起止页码：56

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000457666800007)】；

基金：This work was financially supported by the Project of Marine Public Welfare Profession of China (201405003-2), the Modern Agro-industry Technology Research System (CARS-50-G07) and the Fundamental Research Funds for the Central Universities (222201717019).

语种：英文

外文关键词：Vibrio harveyi; Inactivated vaccine; Adjuvant; Efficient protection

摘要：Turbot (Scophthalmus maximus L.) is a commercially important fish species in China. Despite of its great economic potential, fish farms often suffer severe economic losses due to certain fish diseases. Vaccination has become a common strategy to prevent diseases caused by pathogens in aquaculture industry. However, no inactivated vaccine against Vibrio harveyi of turbot has been reported so far. In this study, we developed an inactivated vaccine using formalin-killed cells of V. harveyi and the efficacy of a commercial adjuvant Montanide (TM) ISA 763 A VG on the inactivated vaccine was evaluated. We found that with an optimum vaccine dosage at 1.0 x 10(8) CFU/fish, a high relative percent survival (RPS) more than 75% was observed at 4 weeks post vaccination (w.p.v.). Moreover, enhanced antibody titer, lysozyme activity, total serum protein and antibacterial property in sera of vaccinated fish were observed at 4, 8, 12 and 16 w.p.v. In conclusion, we developed an efficient inactivated vaccine against V. harveyi in turbot, which not only induced humoral immunity, but also enhanced initial innate immune response for long-term protection.