文献类型：期刊文献

英文题名：Sandwich-type electrochemical aptasensor based on hollow mesoporous carbon spheres loaded with porous dendritic Pd@Pt nanoparticles as signal amplifier for ultrasensitive detection of cardiac troponin I

作者：Wang, Zhenxing[1];Zhao, Hongli[1];Chen, Kaicha[1];Li, Hongyuan[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2021

卷号：1188

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20214411091244);WOS:【SCI-EXPANDED(收录号:WOS:000723226100007)】；

基金：This work was supported by the Science and Technology Com-mission of Shanghai Municipality (STCSM, 20520712500) , and the Fundamental Research Funds for the Central Universities (JKJ01211718) .

语种：英文

外文关键词：Cardiac troponin I; Aptasensor; Pd@Pt bimetallic nanoparticles; Hollow mesoporous carbon spheres; Signal amplification

摘要：Signal amplification is crucial to improve the sensitivity for the electrochemical detection of cardiac troponin I (cTnI), one of the ideal biomarkers for early acute myocardial infarction (AMI) diagnosis. Herein, we developed a novel signal amplification strategy to construct a sandwich-type electrochemical aptasensor for the detection of cTnI. Core-shell Pd@Pt dendritic bimetallic nanoparticles loaded on melamine modified hollow mesoporous carbon spheres (Pd@Pt DNs/NH2-HMCS) was prepared as labels to conjugate with thiol-modification DNA aptamers probe for signal amplification. While introducing numerous amino groups, the melamine functionalized hollow mesoporous carbon spheres (NH2-HMCS) retained the edge-plane-like defective sites for the adhesion and electrocatalytic reduction of H2O2. With the unique characteristics of NH2-HMCS, it not only enhanced the dispersity and loading capacity of core shell Pd@Pt dendritic bimetallic nanoparticles (Pd@Pt DNs), but also improved the stability of bonding by the affinity interaction between Pd@Pt DNs and amino groups of melamine. Meanwhile, the synergistic catalysis effect between Pd@Pt DNs and NH2-HMCS significantly enhanced the electrocatalytic reduction of H2O2 and further amplified the signal. Under optimal conditions, this recommended aptasensor for cTnI detection displayed a wide dynamic range from 0.1 pg/mL to 100.0 ng/mL and a low detection limit of 15.4 fg/mL (S/N = 3). The sensor also successfully realized the analysis of cTnI-spiked human serum samples, meaning potential applications in AMI diagnosis. (C) 2021 Elsevier B.V. All rights reserved.