文献类型：期刊文献

英文题名：Chemically bonded maltose via click chemistry as stationary phase for HILIC

作者：Fu, Qing[1]; Guo, Zhimou[1]; Liang, Tu[2]; Zhang, Xiuli[1]; Xu, Qing[1]; Liang, Xinmiao[1,2]

机构：[1] Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Science, 457 Zhongshan Road, Dalian 116023, China; [2] Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2010

卷号：2

期号：3

起止页码：217

外文期刊名：Analytical Methods

收录：EI(收录号：20103013100399)

语种：英文

外文关键词：Hydrophilicity - Liquid chromatography - Synthesis (chemical) - Maltose - Hydrogen bonds - Silica

摘要：A HILIC stationary phase was synthesized through bonding maltose on silica particles via click chemistry. A set of representative polar compounds including sugars, amino acids and small peptides were employed to evaluate chromatographic properties of Click Maltose. The results illustrated the surface adsorption mechanism from the direct hydrogen bonding interaction between sugars and Click Maltose played an important role on retention behavior of sugars under HILIC mode. The chromatographic behavior of anions on Click Maltose was consistent with HILIC/WAX mechanism, while the retention of cations was depended on electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) mechanism. The Click Maltose stationary phase was successfully used in the separation of standard samples of polar compounds and complex samples of oligosaccharides from the natural product. The Click Maltose also exhibited good reproducibility and stability, which were helpful to further application. Our study confirmed the advantages and application potential of bonded oligosaccharides for HILIC separation. ? 2010 The Royal Society of Chemistry.