文献类型：期刊文献

英文题名：Extracellular Matrix-Mimetic Immunomodulatory Hydrogel for Accelerating Wound Healing

作者：Wang, Honglei[1];Huang, Runzhi[2];Bai, Long[3];Cai, Yixin[1];Lei, Miao[1];Bao, Chunyan[4];Lin, Shaoliang[1];Ji, Shizhao[2];Liu, Changsheng[1];Qu, Xue[1,5,6]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]Second Mil Med Univ, Changhai Hosp, Inst Burns, Dept Burn Surg, Shanghai 200433, Peoples R China;[3]Shanghai Univ, Inst Translat Med, Organoid Res Ctr, Shanghai 200444, Peoples R China;[4]East China Univ Sci & Technol, Inst Fine Chem Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[5]Shanghai Univ, Wenzhou Inst, Wenzhou 325000, Peoples R China;[6]Shanghai Frontier Sci Ctr Optogenet Tech Cell Meta, Shanghai 200237, Peoples R China

年份：2023

卷号：12

期号：27

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20232714359143);WOS:【SCI-EXPANDED(收录号:WOS:001022719600001)】；

基金：H.L.W., R.Z.H., and L.B. contributed equally to this work. This research was supported by the National Key Research and Development Program (2021YFB3800800), National Natural Science Foundation of China (31922041, 32171341, 11932012, 81971836), the 111 Project (B14018), Shanghai Rising-Star Program (Sailing Special Program, No. 23YF1458400), the Science and Technology Innovation Project and Excellent Academic Leader Project of Shanghai Science and Technology Committee (21S31901500, 21XD1421100, 23XD1425000), Deep Blue Talent Project of Naval Medical university, 234 Academic Climbing Program of Changhai hospital.

语种：英文

外文关键词：cell adhesion; dynamic hydrogels; immunomodulation; macrophage polarization; wound healing

摘要：Macrophages play a crucial role in the complete processes of tissue repair and regeneration, and the activation of M2 polarization is an effective approach to provide a pro-regenerative immune microenvironment. Natural extracellular matrix (ECM) has the capability to modulate macrophage activities via its molecular, physical, and mechanical properties. Inspired by this, an ECM-mimetic hydrogel strategy to modulate macrophages via its dynamic structural characteristics and bioactive cell adhesion sites is proposed. The LZM-SC/SS hydrogel is in situ formed through the amidation reaction between lysozyme (LZM), 4-arm-PEG-SC, and 4-arm-PEG-SS, where LZM provides DGR tripeptide for cell adhesion, 4-arm-PEG-SS provides succinyl ester for dynamic hydrolysis, and 4-arm-PEG-SC balances the stability and dynamics of the network. In vitro and subcutaneous tests indicate the dynamic structural evolution and cell adhesion capacity promotes macrophage movement and M2 polarization synergistically. Comprehensive bioinformatic analysis further confirms the immunomodulatory ability, and reveals a significant correlation between M2 polarization and cell adhesion. A full-thickness wound model is employed to validate the induced M2 polarization, vessel development, and accelerated healing by LZM-SC/SS. This study represents a pioneering exploration of macrophage modulation by biomaterials' structures and components rather than drug or cytokines and provides new strategies to promote tissue repair and regeneration.