文献类型：期刊文献

英文题名：Pyrethrum extract induces oxidative DNA damage and AMPK/mTOR-mediated autophagy in SH-SY5Y cells

作者：Yang, Yun[1];Zhang, Yang[1];Gao, Jufang[2];Xu, Wenping[1];Xu, Zhiping[1];Li, Zhong[1];Cheng, Jiagao[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

年份：2020

卷号：740

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20202508848981);WOS:【SCI-EXPANDED(收录号:WOS:000562379100013)】；

基金：This work was financially supported by National Key Research and Development Plan (2017YFD0200300).

语种：英文

外文关键词：Neuroblastoma; Cell viability; DNA damage; Autophagy; Autophagosomes

摘要：Pyrethrum extract is used to produce the most widely applied botanical pesticides in agriculture. Though it primarily targets voltage-gated sodium channels in pests, its toxic effects in non-target systems, particularly in humans, is unclear. In this study, we investigated potential cytotoxic effects and their underlying mechanisms on human nerve cells in vitro. We found that pyrethrum extract exposure markedly inhibited cell viability and triggered oxidative DNA damage in human SH-SY5Y cells. It also induced LC3-II formation, upregulated Beclin-1 protein production, downregulated p62 protein production, and facilitated the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and mechanistic target of rapamycin (mTOR).These results indicate that cytotoxic exposure to pyrethrum extract could be associated with AMPK/MTOR-mediated autophagy in human nerve cells. Furthermore, the oxidative DNA damage suggests that pyrethrum extract exerts severe toxic effects on human nerve cells. In conclusion, pyrethrum extract carries a risk to human health by inducing cytotoxicity. (C) 2020 Elsevier B.V. All rights reserved.