文献类型：期刊文献

中文题名：拮抗青枯劳尔氏菌的荧光假单胞菌SN15-2分离鉴定及其生防能力分析

英文题名：Isolation and identification of bacterium strain SN15-2 against Ralstonia solanacearum and analysis on its bio-control ability

作者：娄海博[1];王晓冰[1];陈俊[1];王伟[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2019

卷号：39

期号：3

起止页码：12

中文期刊名：中国植保导刊

外文期刊名：China Plant Protection

收录：北大核心:【北大核心2017】；

基金：国家重点研发计划(2017YFD0200400);上海市科技兴农重点攻关项目(沪农科创字(2018)第2-5号)

语种：中文

中文关键词：青枯劳尔氏菌;荧光假单胞菌;拮抗作用;定殖能力;生防效果

外文关键词：Ralstonia solanacearum;Pseudomonas fluorescens;antagonistic effect;colonization;bio-control efficiency

摘要：为了筛选出对番茄青枯病具有较好防效的生防菌,采用皿内测定法从上海地区的番茄青枯病自然衰退土壤中,分离到一株对番茄青枯病有很强抑制作用的菌株SN15-2,并进行了分子鉴定和对荧光假单胞菌SN15-2产抗生素能力和定殖能力测定。结果表明,菌株SN15-2为荧光假单胞菌(Pseudomonas fluorescens)。其菌株能产生2,4-二乙酰基间苯三酚(2,4-DAPG)、硝吡咯菌素(PRN)、藤黄绿脓菌素(PLT)。同时,可以产生HCN、噬铁素,能够形成生物膜。SN15-2在施入番茄根际后的前20 d定殖数量减少,20 d后基本稳定,60 d时,在干土中的定殖数量可达3.67×105cfu/g。盆栽防效分析表明,荧光假单胞菌SN15-2对番茄青枯病防效达到46.58%。
To select bio-controlling bacterium with better controlling efficiency against tomato bacterial wilt, SN15-2 was isolated by in-vessel test methods from natural decayed soil of tomato bacterial wilt collected in Shanghai area. SN15-2 was identified as Pseudomonas fluorescens by molecular method, and its antibiotic production and colonization ability were determined. The results showed that it could produce 2,4-two acetyl benzene three phenol(2,4-DAPG), pyrrolnitrin(PRN), pyoluteorin(PLT), HCN, siderophore and biofilm. The number of P. fluorescens SN15-2 colonization decreased in the first 20 days and then remained stable. At the time of 60 days, the colonization numbers of P. fluorescens SN15-2 in the tomato rhizosphere was still 3.67×105 CFU/g(dry soil). The results indicated that P. fluorescens SN15-2 had a good ability of rhizosphere colonization. In green house experiment, the bio-control efficiency of P. fluorescens SN15-2 against tomato bacterial wilt was 46.58%.