文献类型：期刊文献

英文题名：In-Situ Vacuum Distillation of Ethanol Helps To Recycle Cellulase and Yeast during SSF of Delignified Corncob Residues

作者：Zhang, Jian[1];Lei, Cheng[1];Liu, Gang[1];Bao, Yanwen[1];Balan, Venkatesh[2,3,4];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Michigan State Univ, Dept Chem Engn & Mat Sci, E Lansing, MI 48824 USA;[3]Michigan State Univ, Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA;[4]Univ Houston, Sch Technol, Biotechnol Div, Dept Engn Technol, 4800 Calhoun Rd, Houston, TX 77004 USA

年份：2017

卷号：5

期号：12

起止页码：11676

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20180904833978);WOS:【SCI-EXPANDED(收录号:WOS:000417341900061)】；

基金：This research was supported by Natural Science Foundation of China (21306048), the Fundamental Research Funds for the Central Universities of China (WF1514325), the Chinese Scholarship Council (CSC 201506745002), and the National Key Research and Development Program of China (2017YFB0309302). Co-author V.B. thanks the Great Lakes Bioenergy Research Center (http://www.greatlakes-bioenergy.org/) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through Cooperative Agreement DEFC02-07ER64494 between the Board of Regents of the University of Wisconsin System and the U.S. Department of Energy for supporting his work. V.B. thanks the University of Houston and the State of Texas for startup funds.

语种：英文

外文关键词：Delignified corncob residues (DCCR); Enzyme recycling; Yeast recycling; Vacuum distillation; Ethanol; Microbial fermentation; Economic analysis; Biofuel

摘要：The high cost of the lignocellulose-degrading enzyme is one of the major bottlenecks for economically producing cellulosic ethanol. To overcome this bottleneck, we performed in-situ vacuum distillation (at 50 degrees C, 77 mmHg for 30 min) to remove ethanol after simultaneous saccharification and fermentation (SSF) of delignified corncob residues (DCCR). The cellulase and yeast cells left in the stillage were reused by adding fresh DCCR to start a new cycle. This process cycle was repeated five times. Both cellulase and yeast cells were reused five times with a significant cellulase reduction from 16.3 mg of enzyme protein (EP)/g cellulose to as low as 3.3 mg EP/g cellulose. Meanwhile the ethanol in the fermentation broth was maintained as high as 40 g/L before each vacuum distillation. The final ethanol yield after five cycles reached 72.7%. The process economic analysis based on the Aspen Plus model indicated that the cost of the saved cellulase was much higher than that of the additional energy needed to carry out the vacuum distillation. Almost 24% of the ethanol production cost could be reduced by using this new approach compared to batch SSF without cellulase recycling.