文献类型：期刊文献

英文题名：Roles of LuxR in regulating extracellular alkaline serine protease A, extracellular polysaccharide and mobility of Vibrio alginolyticus

作者：Rui, Haopeng[1];Liu, Qin[1];Ma, Yue[1];Wang, Qiyao[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：285

期号：2

起止页码：155

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000257659100003)】；

语种：英文

外文关键词：Vibrio alginolyticus; quorum sensing; LuxR(val); ProA; collagenase; extracellular protease

摘要：In marine Vibrio species, the Vibrio harveyi-type LuxR protein, a key player in a quorum-sensing system, controls the expression of various genes. In this study, the luxR homologue in Vibrio alginolyticus was identified and named luxR(val), whose expression was greatly induced by the increase of cell number. The luxR(val) in-frame deletion mutant showed a significant downregulation of total extracellular protease activity, and especially caused a 70% decrease in the transcript levels of extracellular alkaline serine protease A (proA), which was an important virulent factor of V. alginolyticus. Complementation in trans with luxR(val) could restore the expression of proA to the level of the wild-type strain. Deletion of the luxR(val) gene also resulted in changes of colony morphology, extracellular polysaccharide production and mobility. Therefore, another member of the V. harveyi-type LuxR regulator family has been characterized in V. alginolyticus.