文献类型：期刊文献

英文题名：Target-Specific Imaging of Transmembrane Receptors Using Quinonyl Glycosides Functionalized Quantum Dots

作者：Ma, Wei[1,2];Liu, Hui-Ting[1,2];He, Xiao-Peng[1,2];Zang, Yi[3];Li, Jia[3];Chen, Guo-Rong[1,2];Tian, He[1,2];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Natl Ctr Drug Screening,State Key Lab Drug Res, Shanghai 200031, Peoples R China

年份：2014

卷号：86

期号：11

起止页码：5502

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20142417808620);WOS:【SCI-EXPANDED(收录号:WOS:000336953100043)】；

基金：This research was supported by the 973 Program (Grants 2013CB733700 and 2014CB748500), the National Natural Science Foundation of China (Grants 2115522, 21176076, 21202045, and 21305045), the Fundamental Research Funds for the Central Universities (Grant WJ1313004), the National Key Science & Technology Specific Projects (Grant 012ZX09301-001-004), and the Key Project of Shanghai Science and Technology Commission (Grant 13NM1400900).

语种：英文

外文关键词：Sugars - Cancer cells - Cell culture - Ligands - Biosensors - Proteins - Semiconductor quantum dots

摘要：Here, we describe a novel "switch-on" biosensor based on quinonyl glycosides functionalized quantum dots (QDs) for the specific targeting and imaging of transmembrane glycoprotein receptors on the surface of cancer cells. The design of the quinonyl glycosides lies in that the quinone moiety serves as a quencher of QDs and the glycoside moiety as a biospecific ligand for targeting a receptor. We observed that the quenched photoluminescence of the quinone glycosides functionalized QDs could be significantly recovered by a specific lectin that selectively binds to the glycosides clustering the QDs but was not affected by a panel of nonspecific lectins. Moreover, we determined that quinonyl galactoside functionalized QDs could optically image the asialoglycoprotein receptors of a hepatoma cell line in a target-specific manner. This system might provide new insights into the fabrication of photoluminogenic biosensors for the analysis of the universal ligand receptor recognitions in nature.