文献类型：期刊文献

英文题名：Global Regulator AdpA_1075 Regulates Morphological Differentiation and Ansamitocin Production in Actinosynnema pretiosum subsp. auranticum

作者：Guo, Siyu[1];Leng, Tingting[1];Sun, Xueyuan[1];Zheng, Jiawei[1];Li, Ruihua[1];Chen, Jun[1];Hu, Fengxian[1];Liu, Feng[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：9

期号：11

外文期刊名：BIOENGINEERING-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000894326100001)】；

基金：This work was supported by the National Natural Science Fund for Young Scholars (32001035) and Shanghai Super postdoctoral program 2020.

语种：英文

外文关键词：Actinosynnema pretiosum; ansamitocin P-3; ssgA; AdpA; morphological differentiation

摘要：Actinosynnema pretiosum is a well-known producer of maytansinoid antibiotic ansamitocin P-3 (AP-3). Growth of A. pretiosum in submerged culture was characterized by the formation of complex mycelial particles strongly affecting AP-3 production. However, the genetic determinants involved in mycelial morphology are poorly understood in this genus. Herein a continuum of morphological types of a morphologically stable variant was observed during submerged cultures. Expression analysis revealed that the ssgA_6663 and ftsZ_5883 genes are involved in mycelial aggregation and entanglement. Combing morphology observation and morphology engineering, ssgA_6663 was identified to be responsible for the mycelial intertwining during liquid culture. However, down-regulation of ssgA_6663 transcription was caused by inactivation of adpA_1075, gene coding for an AdpA-like protein. Additionally, the overexpression of adpA_1075 led to an 85% increase in AP-3 production. Electrophoretic mobility shift assays (EMSA) revealed that AdpA_1075 may bind the promoter regions of asm28 gene in asm gene cluster as well as the promoter regions of ssgA_6663. These results confirm that adpA_1075 plays a positive role in AP-3 biosynthesis and morphological differentiation.