文献类型：期刊文献

中文题名：Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies

作者：Suying Liu[1,2,3];Jiaofang Huang[4];Chen Zhang[5];Lihua Wang[6];Chunhai Fan[7];Chao Zhong[3,8,9]

机构：[1]Division of Physical Biology and Bioimaging Center,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai,China;[2]University of Chinese Academy of Sciences,Beijing,China;[3]School of Physical Science and Technology,ShanghaiTech University,Shanghai,China;[4]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,China;[5]Institute of Physics,Swiss Federal Institute of Technology Lausanne(EPFL),Lausanne,Switzerland;[6]The Interdisciplinary Research Center,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Zhangjiang Laboratory,Shanghai,China;[7]School of Chemistry and Chemical Engineering,Institute of Molecular Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,China;[8]Center for Materials Synthetic Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,China;[9]CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,China

年份：2022

卷号：7

期号：3

起止页码：965

中文期刊名：Synthetic and Systems Biotechnology

外文期刊名：合成和系统生物技术（英文）

收录：Scopus;PubMed

基金：funded by the National Key R&D Program of China(Nos.2020YFA0908100 and 2021YFA0910800);the National Science Fund for Distinguished Young Scholars(No.32125023);supported by grants from the National Natural Science Foundation of China(No.31872728);the National Science and Technology Major Project of the Ministry of Science and Technology of China(2020YFA0908900);the Science and Technology Commission of Shanghai Municipality(Nos.19ZR1477100 and 22ZR1416000)for J.F.Huang.

语种：英文

中文关键词：Bacillus subtilis;Biofilms;Synthetic biology;Living materials;Environmental tolerance

摘要：Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix,and these biofilms protect microorganisms against harsh environmental conditions.Bacillus subtilis is a widely used experimental species,and its biofilms are used as representative models of beneficial biofilms.Specifically,B.subtilis biofilms are known to be rich in extracellular polymeric substances(EPS)and other biopolymers such as DNA and proteins like the amyloid protein TasA and the hydrophobic protein BslA.These materials,which form an interconnected,cohesive,three-dimensional polymer network,provide the mechanical stability of biofilms and mediate their adherence to surfaces among other functional contributions.Here,we explored how genetically-encoded components specifically contribute to regulate the growth status,mechanical properties,and antibiotic resistance of B.subtilis biofilms,thereby establishing a solid empirical basis for understanding how various genetic engineering efforts are likely to affect the structure and function of biofilms.We noted discrete contributions to biofilm morphology,mechanical properties,and survival from major biofilm components such as EPS,TasA and BslA.For example,EPS plays an important role in maintaining the stability of the mechanical properties and the antibiotic resistance of biofilms,whereas BslA has a significant impact on the resolution that can be obtained for printing applications.This work provides a deeper understanding of the internal interactions of biofilm components through systematic genetic manipulations.It thus not only broadens the application prospects of beneficial biofilms,but also serves as the basis of future strategies for targeting and effectively removing harmful biofilms.