文献类型：期刊文献

英文题名：Asymmetric bioreduction of keto groups of 4-and 5-Oxodecanoic acids/esters with a new carbonyl reductase

作者：Zhang, Chao[1];Pan, Jiang[1];Li, Chun-Xiu[1];Bai, Yun-Peng[1];Xu, Jian-He[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：102

起止页码：35

外文期刊名：CATALYSIS COMMUNICATIONS

收录：;EI(收录号：20173404077696);WOS:【SCI-EXPANDED(收录号:WOS:000413058800009)】；

基金：This work was financially sponsored by the National Key Research and Development of China (2016YFA0204300), National Natural Science Foundation of China (Nos. 21505044, 21536004 & 21776085) Shanghai Pujiang Program (15PJ1401200) and the Fundamental Research Funds for the Central Universities (222201714026).

语种：英文

外文关键词：4-Oxodecanoic acid; 5-Oxodecanoic acid; (R)-gamma-Decalactone; (R)-delta-Decalactone; Carbonyl reductase; Asymmetric reduction

摘要：A novel carbonyl reductase from Serratia marcescens, SmCR, was successfully cloned and overexpressed in Escherichia coil. SmCR could catalyze the asymmetric reduction of long-chain keto acids/esters containing remote carbonyl groups, such as 4-oxo- and 5-oxodecanoic acids, yielding chiral gamma- and delta-decalactones with high en-antiopurity (up to 99% ee). This is the first report of enzymatic synthesis of (R)-gamma- and (R)-delta-decalactones starting from gamma-, delta-keto acids using free enzymes.