文献类型：期刊文献

英文题名：Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence in Vibrio alginolyticus

作者：Tian, Yang[1];Wang, Qiyao[1];Liu, Qin[1];Ma, Yue[1];Cao, Xiaodan[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：190

期号：5

起止页码：585

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000259934500008)】；

基金：We thank Professor Debra Milton (Umea University, Sweden) and Professor Bonnie L. Bassler (Princeton University, USA) for kindly sending strains used for the mutant construction and V. harveyi reporter strain in this study. This work was supported by National Natural Science Foundation of China (No. U0633004) and Shanghai Leading Academic Discipline Project (No. B505).

语种：英文

外文关键词：RpoS; Vibrio alginolyticus; stress; AI-2 synthesis; ECP

摘要：Vibrio alginolyticus, a marine bacterium, is an opportunistic pathogen capable of causing vibriosis with high mortality to fishes in the South China Sea. Stress resistance is very important for its survival in the natural environment and upon infection of the host. RpoS, an alternative sigma factor, is considered as an important regulator involved in stress response and virulence in many pathogens. In this study, the rpoS gene was cloned and characterized to evaluate the role of RpoS in V. alginolyticus. The predicted protein showed high identity with other reported rpoS gene products. The in-frame deleted mutation of rpoS in V. alginolyticus led to sensitivity of the strain to ethanol, hyperosmolarity, heat, and hydrogen peroxide challenges. Further studies showed that extracellular autoinducer 2 level, four of seven detected protease activities, and cytotoxicity of extracellular products were markedly decreased in the rpoS mutant compared with that in the wild-type strain. The results indicated that the global regulator RpoS was part of the regulatory networks of virulence and LuxS quorum sensing system.