文献类型：期刊文献

英文题名：Paper-Based Enzymatic Colorimetric Assay for Rapid Malathion Detection

作者：Li, Jian-Hui[1];Deng, Xue-Lei[2];Zhao, Yu-Lian[2];Zhang, Xiao-Yan[2];Bai, Yun-Peng[2]

机构：[1]Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：193

期号：8

起止页码：2534

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20211410164457);WOS:【SCI-EXPANDED(收录号:WOS:000635058000004)】；

基金：This work was financially sponsored by the National Key Research and Development Program of China (2016YFA0204300), the Natural Science Foundation of Shanghai (18ZR1409900), and the Key Project of the Shanghai Science and Technology Committee (18DZ1112703).

语种：英文

外文关键词：Enzyme immobilization; Paper-based biosensor; Malathion detection; Polymer– enzyme conjugate; Organophosphate hydrolase

摘要：Due to their unique properties, paper-based biosensors have attracted attention as inexpensive devices for on-site analysis. To achieve fast and sensitive detection of analytes, immobilization of enzymes with high apparent activities on paper is highly desirable; however, this is challenging. Herein, we report an improved approach to attach a malathion degrading enzyme, PoOPHM9, on paper via an interlocking network of Pluronic F127 (PF127)-poly(acrylic acid)-enzyme conjugates. The addition of PF127 improved retention of enzymatic activity as the apparent kinetic constant V-max of the immobilized enzyme increased two-fold compared with the paper prepared without PF127. The PF127-poly(acrylic acid)-PoOPHM9 papers provided rapid colorimetric detection of malathion at 0.1-50 mM. The detection was completed within 5 min using a smartphone and image analysis software. As a proof-of-concept, malathion-contaminated water, plant, and apple samples were analyzed with the papers successfully. This material is promising for on-site rapid analysis of malathion-contaminated samples.