文献类型：期刊文献

英文题名：A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila

作者：Qian, Yajie[1,2];Li, Ting[1,2];Zhou, Siyu[1,2];Chen, Xianjun[1,2];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2023

卷号：12

期号：3

起止页码：664

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000948632600001)】；

基金：We thank Xiaoquan Li, Yonggang Ren, Huaxing Zi at Institute of Neuroscience, Chinese Academy of Sciences for technical assistance. We thank Jiulin Du (Institute of Neuroscience, Chinese Academy of Sciences) for providing plasmids Tol2-UAS-GFP. We thank Yang Zhang (East China University of Science and Technology) for providing Drosophila S2 cells. This research was supported by the National Key Research and Development Program of China (2022YFC3400100 to Y.Y. and X.C.; 2019YFA0904800 to Y.Y.) , STI2030-Major Projects (2021ZD0202200 and 2021ZD0202203 to X.C.) , NSFC (32121005, 32150028, 91857202, and 21937004 to Y.Y., 32250009 and 31600688 to X.C., 31901033 to T.L.) , the Shanghai Municipal Education Commission-Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (2021 Sci & Tech 03-28) to Y.Y. and X.C., the Shanghai Rising-Star Program to X.C., the Shanghai Sailing Program (19YF1411300 to T.L.) , the State Key Laboratory of Bioreactor Engineering to Y.Y. and X.C., the Fundamental Research Funds for the Central Universities to Y.Y. and X.C., and the China Postdoctoral Science Foundation (2019M651413 to T.L.) .

语种：英文

外文关键词：optogenetics; single-component; gene expression; Gal4-UAS

摘要：The light-regulated Gal4-UAS system has offered new ways to control cellular activities with precise spatial and temporal resolution in zebrafish and Drosophila. However, the existing optogenetic Gal4-UAS systems suffer from having multiple protein components and a dependence on extraneous light -sensitive cofactors, which increase the technical complexity and limit the portability of these systems. To overcome these limitations, we herein describe the development of a novel optogenetic Gal4-UAS system (ltLightOn) for both zebrafish and Drosophila based on a single light-switchable transactivator, termed GAVPOLT, which dimerizes and binds to gene promoters to activate transgene expression upon blue light illumination. The ltLightOn system is independent of exogenous cofactors and exhibits a more than 2400-fold ON/OFF gene expression ratio, allowing quantitative, spatial, and temporal control of gene expression. We further demonstrate the usefulness of the ltLightOn system in regulating zebrafish embryonic development by controlling the expression of lefty1 by light. We believe that this single-component optogenetic system will be immensely useful in understanding the gene function and behavioral circuits in zebrafish and Drosophila.