文献类型：期刊文献

中文题名：Cu^(2+)、Zn^(2+)胁迫对蛹虫草(Cordyceps militaris)抗氧化酶活性及脂氧化水平的影响

英文题名：The Effect of Cu^(2+) and Zn^(2+) Stress on Antioxidant Enzyme Activity and Lipid Peroxidation Level of Entomogenous Fungal Species Cordyceps Militaris

作者：何卓晶[1];王尊生[1];李素霞[1];袁勤生[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237

年份：2006

卷号：13

期号：1

起止页码：36

中文期刊名：药物生物技术

外文期刊名：Pharmaceutical Biotechnology

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；

基金：上海市重点学科建设项目资助(编号:050716)

语种：中文

中文关键词：超氧化物歧化酶;过氧化氢酶;脂氧化;氧化胁迫;蛹虫草

外文关键词：SOD, CAT, LPO, Oxidative stress, Cordyceps militaris

摘要：研究了Cu2+、Zn2+对蛹虫草菌丝体抗氧化酶活性及脂氧化水平的影响。结果表明:高浓度的Cu2+抑制菌丝体的生长和细胞内抗氧化酶的活性;高浓度的Zn2+对菌丝体的生长也有抑制作用但抗氧化酶的活性变化不大。脂氧化水平随细胞内过氧化状态的变化而变化。蛹虫草菌丝体对Cu2+、Zn2+有较高的耐受度。
The effect of Cu^2＋ and Zn^2＋ stress on antioxidant enzyme activity and membrane lipid peroxi dation level （LPO） of entomogenous fungal species cordyceps militaris was studied, Cu, and Zn containing superoxide dismutase （Cu, Zn-SOD） and catalase （CAT） activity were detectable in the culture filtrates, and intracellular Cu, Zn-SOD and CAT activity as a proportion protein was the highest in early log phase culture, LPO was estimated based on thiobarbituric acid （TBA） reactivity. The results showed that Cu^2＋ excess could restrain culture growth and antioxidant enzyme activity; Zn^2＋ excess also could restrain culture growth, but antioxidant enzyme activity in the ceils appeared to be insensitive to Zn^2＋ excess. The LPO levels in the ceils were relative to the cell oxidative conditions. The strain C. militaris has high resistance to Cu^2＋ and Zn^2＋ , and has high levels of antioxidant enzyme activities.