文献类型：期刊文献

中文题名：A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

英文题名：A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

作者：Yang, Yangyang[1];Zhang, Chen[1];Pan, Rizhao[1];Zhang, Shiwei[1];Yao, Shengtao[1];Tang, Yao[1];Zhu, Weilong[1];Wang, Liyue[1];Zhu, Weiping[1];Xu, Yufang[1];Qian, Xuhong[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：31

期号：1

起止页码：125

中文期刊名：Chinese Chemical Letters

外文期刊名：CHINESE CHEMICAL LETTERS

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000509632800023)】；CSCD:【CSCD2019_2020】；

基金：This work was supported by the Shanghai Sailing Program, National Natural Science Foundation of China (No. 31600802), Science and Technology Commission of Shanghai Municipality (No. 18DZ1112703) and the Fundamental Research Funds for the Central Universities.

语种：英文

中文关键词：Fluorescent probe;Alkaline phosphatase;Ratiometric;ESIPT;Biomarker

外文关键词：Fluorescent probe; Alkaline phosphatase; Ratiometric; ESIPT; Biomarker

摘要：Alkaline phosphatase(ALP)is one of essential biomarkers in mammalian tissue.Here we report a ratiometric probe for ALP,which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide(BTHPB).The enzymatic dephosphorylation converts the probe to BTHPB,which exhibits a large spectral red-shift(120 nm),allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL.The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.
Alkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.