文献类型：期刊文献

英文题名：Application of 8-parallel micro-bioreactor system with non-invasive optical pH and DO biosensor in high-throughput screening of L-lactic acid producing strain

作者：Tian, Xiwei[1];Zhou, Gang[1];Wang, Weifei[1];Zhang, Ming[1];Hang, Haifeng[1];Mohsin, Ali[1];Chu, Ju[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China

年份：2018

卷号：5

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513080820);WOS:【SCI-EXPANDED(收录号:WOS:000461106800020)】；

基金：This work was financially supported by the National Science Foundation for Young Scientists of China (31700038), the National Key Research and Development Program (2017YFB0309302) and the National Major Scientific Technological Special Project (2012YQ15008706).

语种：英文

外文关键词：High-throughput screening; Lactic acid; Lactobacillus paracasei; Micro-bioreactor; Optical biosensor

摘要：Background: Compared with shake flask, bioreactor offers a relatively stable and controllable extracellular environment for cell growth and metabolism. Meanwhile, parallel micro-bioreactor system could well meet the screening flux requirement in the process of high-throughput strain screening. In this study, a self-developed 8-parallel microbioreactor system with non-invasive optical biosensors was introduced to substitute the traditional shake flask. Results: Optical pH and DO biosensors could be well applied for the process monitoring and controlling in the cultures of commonly used microorganisms through maintaining constant temperature and bioreactor shading treatment. Subsequently, 8-parallel micro-bioreactor system was adopted in the rescreening procedure of high-throughput screening process, and it significantly increased the feasibility of scaling up a cultivating system without any sacrifice on the screening flux. As a result, a designated mutant strain Lactobacillus paracasei S4 was obtained, which presented an improvement of 18.9% on titer value of L-lactic acid. Moreover, the yield also increased from 0.903 +/- 0.005 to 0.932 +/- 0.013 g/g. Conclusion: In this study, the micro-bioreactor system proved to be applicable and effective in the rescreening procedure of high-throughput screening process. The adopted approach provided a robust tool for screening the strain with high L-lactic acid producing performance.