文献类型：期刊文献

英文题名：Sensitive and fast assessment of DNA methylation degree by a luminescent lanthanide-functionalized MOF probe

作者：Chen, Guoli[1];Hao, Ji-Na[1];Li, Yongsheng[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Engn Res Ctr Hierarch Nanomat, Sch Mat Sci & Engn, Lab Low Dimens Mat Chem,Key Lab Ultrafine Mat,Mini, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, State Key Lab Incubat Base Green Proc Chem Engn, Shihezi 832003, Peoples R China

年份：2025

卷号：215

外文期刊名：MICROCHEMICAL JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001530400200005)】；

基金：This work was supported by the National Key Research and Development Program of China (2022YFC2403203, 2024YFF0508601), the National Natural Science Foundation of China (52172279), the Leading Talents in Shanghai in 2018, the Key Field Research Program (2023AB054), and the Higher Education Discipline Innovation Project (B14018).

语种：英文

外文关键词：Luminescent probes; Lanthanides; Metal-organic frameworks; Biosensing; DNA methylation

摘要：5-Methylcytosine (5-mC), as one of the primary forms of DNA methylation, plays essential roles in a variety of biological activities. Its rapid and precise detection is therefore paramount for abnormal DNA methylation assessment, but still remains challenging. Herein, a highly luminescent and stable lanthanide-based metalorganic framework (MOF) probe, namely Eu-ZrMOF, is firstly designed for qualitative and quantitative analysis of 5-mC, wherein the organic building units of 1,3,5-benzenetricarboxylic acid (BTC) serve as both the energy donors and recognition molecules, and the modified Eu3+ ions function as intense signal reporters. Significantly, the luminescence of the fabricated Eu-ZrMOF probe can be remarkably quenched by 5-mC via its inner filter effect on the BTC absorption, achieving high response to 5-mC in a very short time (20 s). Moreover, such an efficient, instantaneous response probe presents excellent specificity towards 5-mC among various coexisting species in urine, and high sensitivity with a linear detection range of 0-200 mu M and limit of detection (LOD) of 0.18 mu M, thereby enabling it to be applied in accurately quantifying 5-mC content in real urine samples with high recoveries (95.5-98.5 %) and low relative standard deviations (< 2.5 %). This study demonstrates a simple, fast and effective approach for sensitive identification of urinary 5-mC, prefiguring its great potential for the assessment of DNA methylation associated with genetic diseases and tumors.