文献类型：期刊文献

英文题名：A ROS-ResponsiveDoxorubicin-Loaded Liposomal StrategyReduces Postablation Electrical Conduction Recovery in a CARTO-GuidedCanine Right Atrial Model

作者：Zhuge, Ying[1];Yang, Liping[2];Ge, Yulong[1];Ji, Qingdong[2];Lu, Jing[2];Zheng, Shuhui[2];Wang, Yiting[2];Sun, Mingyue[2];Zou, Jiafeng[2];Wang, Fang[1];Gao, Feng[2]

机构：[1]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Cardiol, Sch Med, Shanghai 200800, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontier Sci Ctr Optogenet Tech Cell Meta, Sch Pharm, Shanghai 200237, Peoples R China

年份：2026

卷号：18

期号：28

起止页码：38662

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20263021174782);Scopus(收录号：2-s2.0-105045569838);WOS:【SCI-EXPANDED(收录号:WOS:001817476000001)】；

基金：This work was financially supported by the Development Program of China (No. 2019YFA0904800), National Key Research and Development Program of China (No. 2023YFB3208200), National Natural Science Foundation of China (No. 82370318), Science and Technology Tackling Project of Shanghai Songjiang District Science and Technology Commission (No. 2024SJKJGG066), Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Research Unit of New Techniques for Live-cell Metabolic Imaging (Chinese Academy of Medical Sciences, No. 2019-I2M-5-013). The author thanks the exceptional experimental environment of the State Key Laboratory of Bioreactor Engineering (Shanghai), the Fundamental Research Funds for the Central Universities. The authors also thank Prof. Yuzheng Zhao (ECUST), Prof. Xianjun Chen (ECUST), and Prof. Minbo Lan (ECUST) for their help.

语种：英文

外文关键词：radiofrequency catheter ablation; ROS-responsive liposome; apoptosis induction; canine models; CARTO system

摘要：Assessment of nanoplatform efficacy in reducing postablation electrical conduction recovery remains a major challenge in the development of controllable adjunctive therapies for radiofrequency catheter ablation (RFCA). Herein, we established a minimally invasive CARTO-guided canine model of right atrial postablation conduction recovery for mechanistic and pharmacodynamic evaluation. Here, a doxorubicin (DOX)-loaded and reactive oxygen species (ROS)-responsive liposome (DOX@rNP) was constructed to respond to high ROS levels, leading to the high local DOX concentration in the right atrium after ablation, which was 2.8-fold higher than that of the healthy group. Subsequently, released DOX induced apoptosis at the ablation site and modulated the Bax/Bcl-2 pathway, with associated effects on cleaved caspase-3, gamma-H2AX, and fibrosis-related remodeling. CARTO-based voltage mapping and pacing assessment showed that DOX@rNP helped maintain postablation conduction block in both the short-term (30 days) and long-term (180 days). Additionally, in vivo electrocardiographic results showed that DOX@rNP treatment was associated with a lower observed electrical conduction recurrence rate than RFCA alone at 180 days (20.0% vs 80.0%), with a significant difference based on binary conduction-outcome analysis using a one-tailed Welch's t-test. This study establishes a large-animal model of postablation electrical conduction recovery and supports a ROS-responsive liposomal strategy for reinforcing ablation-site injury and suppressing lesion reconnection.