文献类型：期刊文献

英文题名：Horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy

作者：Cao, Xiaodan[1];Chen, Chao[1];Yu, Haijun[1];Wang, Ping[1]

机构：[1]E China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：37

期号：1

起止页码：81

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20160801996928);WOS:【SCI-EXPANDED(收录号:WOS:000347028400009)】；

基金：This work was supported by seed grant for cultivating and interdisciplinary research of Chinese Education Ministry, National Natural Science Foundation of China (21303050), China Postdoctoral Science Foundation Grant (2013M540341), and National "Thousand Talents Program" of China.

语种：英文

外文关键词：Breast cancer; Chitosan nanoparticles; Enzyme-prodrug therapy; Horseradish peroxidase; Indole-3-acetic acid; Nanoparticles for cancer therapy; Protein stabilization

摘要：Among various enzyme-based therapies, enzyme-prodrug therapy (EPT) promises minimized side effects in that it activates non-toxic prodrugs locally where the enzymes are placed. The success of such an approach requires high enzyme stability against both structural denaturation and potential immunogenicity. This work examines the efficiency of nanoparticles for enzyme protection in EPT applications. Specifically, horseradish peroxidase (HRP)-encapsulated chitosan nanoparticles (HRP-CSNP) were constructed and examined with respect to stability enhancement. HRP-CSNP retained enzyme activity and had improved stability at 37 A degrees C in the presence of a denaturant, urea. The nanoparticles effectively bound to the surface of human breast cancer cell Bcap37 and led to over 80 % cell death when applied with a prodrug indole-3-acetic acid.