文献类型：期刊文献

英文题名：Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli

作者：Yi, Yulan[1];Sheng, Huakai[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomanufacturing, Shanghai, Peoples R China

年份：2014

卷号：14

外文期刊名：BMC BIOTECHNOLOGY

收录：;EI(收录号：20142517841844);WOS:【SCI-EXPANDED(收录号:WOS:000338143500001)】；

基金：This study was supported by the National High Technology Research and Development Program of China (Grant No's. 2012AA022104 & 2012AA021205), the Fundamental Research Funds for the Central Universities (Grant No's. 222201313007 & 222201314048), the National Special Fund for State Key Laboratory of Bioreactor Engineering (No.2060204).

语种：英文

外文关键词：Trans-4-hydroxy-L-proline; Recombinant Corynebacterium glutamicum; Recombinant Escherichia coli; Proline 4-hydroxylases

摘要：Background: Trans-4-hydroxy-L-proline (trans-Hyp), one of the hydroxyproline (Hyp) isomers, is a useful chiral building block in the production of many pharmaceuticals. Although there are some natural biosynthetic pathways of trans-Hyp existing in microorganisms, the yield is still too low to be scaled up for industrial applications. Until now the production of trans-Hyp is mainly from the acid hydrolysis of collagen. Due to the increasing environmental concerns on those severe chemical processes and complicated downstream separation, it is essential to explore some environment-friendly processes such as constructing new recombinant strains to develop efficient process for trans-Hyp production. Result: In this study, the genes of trans-proline 4-hydroxylase (trans-P4H) from diverse resources were cloned and expressed in Corynebacterium glutamicum and Escherichia coli, respectively. The trans-Hyp production by these recombinant strains was investigated. The results showed that all the genes from different resources had been expressed actively. Both the recombinant C. glutamicum and E. coli strains could produce trans-Hyp in the absence of proline and 2-oxoglutarate. Conclusions: The whole cell microbial systems for trans-Hyp production have been successfully constructed by introducing trans-P4H into C. glutamicum and E. coli. Although the highest yield was obtained in recombinant E. coli, using recombinant C. glutamicum strains to produce trans-Hyp was a new attempt.