文献类型：期刊文献

英文题名：Constructing xylose-assimilating pathways in Pediococcus acidilactici for high titer D-lactic acid fermentation from corn stover feedstock

作者：Qiu, Zhongyang[1];Gao, Qiuqiang[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：245

起止页码：1369

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20172403762428);WOS:【SCI-EXPANDED(收录号:WOS:000413038300009)】；

语种：英文

外文关键词：Pediococcus acidilactici; Xylose-assimilating pathway; D-Lactic acid; Adaptive evolution; Lignocellulose

摘要：Xylose-assimilating pathway was constructed in a D-lactic acid producing Pediococcus acidilactici strain and evolutionary adapted to yield a co-fermentation strain P. acidilactici ZY15 with 97.3 g/L of D-lactic acid and xylose conversion of 92.6% obtained in the high solids content simultaneous saccharification and co-fermentation (SSCF) of dry dilute acid pretreated and biodetoxified corn stover feedstock. The heterologous genes encoding xylose isomerase (xylA) and xylulokinase (xylB) were screened and integrated into the P. acidilactici chromosome. The metabolic flux to acetic acid in phosphoketolase pathway was re-directed to pentose phosphate pathway by substituting the endogenous phosphoketolase gene (pkt) with the heterologous transketolase (tkt) and transaldolase (tal) genes. The xylose-assimilating ability of the newly constructed P. acidilactici strain was significantly improved by adaptive evolution. This study provided an important strain and process prototype for high titer D-lactic acid production from lignocellulose feedstock with efficient xylose assimilation. (C) 2017 Elsevier Ltd. All rights reserved.