文献类型：期刊文献

英文题名：A hyperbranched polyethylenimine functionalized stationary phase for hydrophilic interaction liquid chromatography

作者：Peng, Yahui[1];Hou, Yanjie[1];Zhang, Feifang[1];Shen, Guobin[1];Yang, Bingcheng[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, 267 BO,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：408

期号：13

起止页码：3633

外文期刊名：ANALYTICAL AND BIOANALYTICAL CHEMISTRY

收录：;EI(收录号：20161102103999);WOS:【SCI-EXPANDED(收录号:WOS:000374658400025)】；

基金：The research was sponsored by the National Natural Science Foundation of China (No. 21322502, 21477037). It also thanks for the support from outstanding young talent cultivation fund of ECUST.

语种：英文

外文关键词：Stationary phase; Polyethylenimine; Hyperbranched; Hydrophilic interaction chromatography; Ion chromatography

摘要：A hyperbranched stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared by grafting polyethylenimine (PEI) onto silica gel (termed as PEI-Sil). Rich primary, secondary, and tertiary amino groups associated with PEI render its good hydrophility. More importantly, the hyperbranched structure of PEI molecule is greatly helpful in improving interaction with polar analytes. For several kinds of model polar compounds, including organic acids, nucleosides, nucleic acid bases, amino acids, cephalosporins, and non-reducing sugars, PEI-Sil demonstrated excellent separation performance in terms of running stability, reproducibility, and separation efficiency (e.g., plate count similar to 74,000/m). In addition, PEI-Sil also exhibited much better separation selectivity toward inorganic anions when operated in the mode of ion chromatography relative to a commercial amino propyl-bonded column.