文献类型：期刊文献

英文题名：All-in-one microfluidic device for on-site diagnosis of pathogens based on an integrated continuous flow PCR and electrophoresis biochip

作者：Li, Zhenqing[1];Ju, Ruixue[1];Sekine, Shinichi[2];Zhang, Dawei[1];Zhuang, Songlin[1];Yamaguchi, Yoshinori[3,4]

机构：[1]Univ Shanghai Sci & Technol, Engn Res Ctr Opt Instrument & Syst, Shanghai Key Lab Modern Opt Syst, Minist Educ,Key Lab Opt Instruments & Equipment M, Shanghai, Peoples R China;[2]Osaka Univ, Grad Sch Dent, Dept Prevent Dent, Osaka, Japan;[3]East China Univ Sci & Technol, Grad Sch Sci, Inst Photon & Biomed IPBM, Shanghai, Peoples R China;[4]Osaka Univ, Dept Appl Phys, Grad Sch Engn, Osaka, Japan

年份：2019

卷号：19

期号：16

起止页码：2663

外文期刊名：LAB ON A CHIP

收录：;EI(收录号：20201708538525);WOS:【SCI-EXPANDED(收录号:WOS:000478908200010)】；

基金：This work was supported by the National Key Research and Development Program of China (2016YFD0500603), Science and Technology Commission of Shanghai Municipality (China) (No. 18441900400) and National Natural Science Foundation of China (No. 61775140). We also gratefully acknowledge financial support from the University of Shanghai for Science and Technology (No. 2017KJFZ049).

语种：英文

外文关键词：Microfluidics - Polymerase chain reaction - Contamination - Biochips - Diagnosis - Syringes - Fluidic devices

摘要：Current continuous flow polymerase chain reaction (CF-PCR) microfluidic chips require external precision syringe pumps and off-line methods (e.g., electrophoresis and hybridization) to detect PCR products, resulting in complex operations and possible cross-contamination and consequently CF-PCR is still confined to laboratories. Herein, a portable all-in-one microfluidic device is fabricated for rapid diagnosis of pathogens based on an integrated CF-PCR and electrophoresis biochip. A new method was proposed for automatic sample injection into the chip which can substitute the costly external precision syringe pump. It not only achieves rapid DNA amplification and on-site PCR product detection, but also realizes automatic sample injection. As an application, three periodontal pathogens (e. g., Porphyromonas gingivalis, Treponema denticola and Tannerela forsythia) were successfully amplified in the device. Treponema denticola was amplified in as short as 2' 31", and detection of PCR products was completed within 3' 43". The minimum number of bacteria that can be amplified was 125 cfu per mu l. The all-in-one device has the potential to be applied in point-of-care nucleic acid testing for diseases.