文献类型：期刊文献

英文题名：Characterization of an Lrp/AsnC family regulator SCO3361, controlling actinorhodin production and morphological development in Streptomyces coelicolor

作者：Liu, Jing[1];Li, Jie[1];Dong, Hong[1];Chen, Yunfu[1];Wang, Yansheng[1];Wu, Hang[1];Li, Changrun[1];Weaver, David T.[1];Zhang, Lixin[1,2];Zhang, Buchang[1]

机构：[1]Anhui Univ, Inst Hlth Sci, Sch Chem & Chem Engn, Sch Life Sci, Hefei 230601, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：101

期号：14

起止页码：5773

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20172403769660);WOS:【SCI-EXPANDED(收录号:WOS:000405181100018)】；

基金：This work was supported by the National Program on Key Basic Research Project (973 program, 2013CB734000), the National Natural Science Foundation of China (Grant Nos. 31300081, 31570074, 31600064), the Anhui Provincial Natural Science Foundation (Grant No. 1708085QC49), and the Initial Foundation of Doctoral Scientific Research in Anhui University (01001904, J01001935).

语种：英文

外文关键词：Lrp/AsnC family; Streptomyces coelicolor; SCO3361; Act production; Morphological development; Interactional regulation

摘要：Lrp/AsnC family regulators have been found in many bacteria as crucial regulators controlling diverse cellular processes. By genomic alignment, we found that SCO3361, an Lrp/AsnC family protein from Streptomyces coelicolor, shared the highest similarity to the SACE_Lrp from Saccharopolyspora erythraea. Deletion of SCO3361 led to dramatic reduction in actinorhodin (Act) production and delay in aerial mycelium formation and sporulation on solid media. Dissection of the mechanism underlying the function of SCO3361 in Act production revealed that it altered the transcription of the cluster-situated regulator gene actII-ORF4 by directly binding to its promoter. SCO3361 was an auto-regulator and simultaneously activated the transcription of its adjacent divergently transcribed gene SCO3362. SCO3361 affected aerial hyphae formation and sporulation of S. coelicolor by activating the expression of amfC, whiB, and ssgB. Phenylalanine and cysteine were identified as the effector molecules of SCO3361, with phenylalanine reducing the binding affinity, whereas cysteine increasing it. Moreover, interactional regulation between SCO3361 and SACE_Lrp was discovered for binding to each other's target gene promoter in this work. Our findings indicate that SCO3361 functions as a pleiotropic regulator controlling secondary metabolism and morphological development in S. coelicolor.