文献类型：期刊文献

英文题名：Enhanced production of biosynthesized lycopene via heterogenous MVA pathway based on chromosomal multiple position integration strategy plus plasmid systems in Escherichia coli

作者：Wei, Yanlong[1];Mohsin, Ali[1];Hong, Qi[1];Guo, Meijin[1];Fang, Hongqing[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Anhui Univ, Inst Hlth Sci, Econ & Technol Dev Zone, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China;[3]Acad Mil Med Sci, Inst Biotechnol, 20 Dongda St, Beijing 100071, Peoples R China

年份：2018

卷号：250

起止页码：382

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20175004520113);WOS:【SCI-EXPANDED(收录号:WOS:000430740000047)】；

基金：This research was supported by grants from the National Natural Science Foundation of China (81373286), National Basic Research Program of China (2011CBA00800), and Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Lycopene biosynthesis; Heterogenous MVA pathway; Chromosomal multiple position integration strategy; Plasmid system; Escherichia coli

摘要：The multiple plasmid system, mostly relied, for heterogeneous gene expression, results in genetic instability and low mean productivity. To address this, an integration method was employed for investigating expression of heterogenous pathway in E. coli cells; where mevalonate upper pathway was found efficiently expressed. Subsequently, to improve lycopene production, chromosomal multiple position integration strategy was used to strengthen mevalonate upper pathway. Meanwhile, the plasmid system was employed for mevalonate lower pathway and lycopene pathway expression to finally generate the mutant D711 strain. Comparatively, highest level of 68.5 mg/L lycopene was produced by D711 outperforming its maximum average productivity of 2.85 mg/L/h with over 2-folds enhancement. In addition, lycopene level was almost 224 mg/L after optimization of induction time, which was 3.3-fold higher than standard control condition. Finally, expression Performance Parameter was developed for scoring mutants and evaluating these two strategies, indicating chromosomal multiple position integration strategy as more efficient approach.