文献类型：期刊文献

英文题名：Nitroso-Caged Rhodamine: A Superior Green Light-Activatable Fluorophore for Single-Molecule Localization Super-Resolution Imaging

作者：Zheng, Ying[1];Ye, Zhiwei[1];Liu, Zengjin[2];Yang, Wei[1];Zhang, Xinfu[1];Yang, Youjun[3];Xiao, Yi[1]

机构：[1]Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China;[2]Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Drug Res Ctr Integrated Tradit Chinese & Western, Luzhou 646000, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2021

卷号：93

期号：22

起止页码：7833

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20212610546344);WOS:【SCI-EXPANDED(收录号:WOS:000661465300010)】；

基金：This work was supported by the National Natural Science Foundation of China (nos. 21421005, 21776037, 21804016, and 22004011), China Postdoctoral Science Foundation (nos. BX20200073 and 2020M670754), Fundamental Research Funds for the Central Universities (No. DUT20JC39) and Dalian Science and Technology Innovation Fund (no. 2020JJ25CY014). We are thankful for the directions from Prof. Xiaojun Peng at DLUT and Prof. Xuhong Qian at ECUST. The fluorescent imaging was performed with support from the Chemical Analysis and Research Center, Dalian University of Technology.

语种：英文

外文关键词：Probes - Optical resolving power - Cytology - Laser excitation - Cells - Molecules - Additives

摘要：The evolution of super-resolution imaging techniques, especially single-molecule localization microscopy, demands the engineering of switchable fluorophores with labeling functionality. Yet, the switching of these fluorophores depends on the exterior conditions of UV light and enhancing buffers, which is bioincompatible for living-cell applications. Herein, to surpass these limitations, a nitroso-caging strategy is employed to cage rhodamines into leuco forms, which for the first time, is discovered to uncage highly bright zwitterions by green light. Further, clickable construction grants the specificity and versatility for labeling various components in living cells. The simultaneous photoactivation and excitation of these novel probes allow for single-laser super-resolution imaging without any harmful additives. Super-resolution imaging of microtubules in fixed cells or mitochondria and the distribution of glycans and H2B proteins in living cells are achieved at a molecular scale with robust integrity. We envision that our nitroso-caging probes would set a platform for the development of future visible-activatable probes.