文献类型：期刊文献

英文题名：Graphene Oxide-Based Fluorescent "on/off" Switch for Visual Bioassay Using "Molecular Beacon"-Hosted Hoechst Dyes

作者：Zhang, Min[1];Huynh-Nhu Le[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：5

期号：17

起止页码：8278

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20133916769515);WOS:【SCI-EXPANDED(收录号:WOS:000330017100006)】；

基金：This study is supported by the China NSF (21075040, 21335003), the Shanghai Fund (11 nm0502500), SRFDP (20120074110009), and the Key Grant Project (313019) of Chinese Ministry of Education, and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：graphene; Hoechst; molecular beacon; biosensing; exonuclease

摘要：In this study, the fluorescence of Hoechst dyes is significantly lit up by addition of our designed MB probe, forming a complex of "molecular beacon"-hosted Hoechst dyes (HMB). Combined with this property, a novel graphene oxide (GO)-based fluorescent "on/off" switch was developed to visually follow bioassay utilizing HMB as signal indicators and GO as an excellent energy acceptor to efficiently quench the fluorescence of HMB in a label-easy format. We have demonstrated its application for label-easy fluorescence "turn on" detection of sequence-specific DNA and "turn off" detection of exonuclease with sensitivity and selectivity in a single step in homogeneous solution. Compared to traditional molecular beacons, the proposed design is cost-effective and simple to prepare without fluorescence labeling or chemical modification.