文献类型：期刊文献

英文题名：Chitooligosaccharides-modified PLGA nanoparticles enhance the antitumor efficacy of AZD9291 (Osimertinib) by promoting apoptosis

作者：Hu, Xiaochao[1];Chen, Shumin[1];Yin, Hao[2];Wang, Qi[1];Duan, Yourong[3];Jiang, Lihua[1];Zhao, Liming[1,4]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, R&D Ctr Separat & Extract Technol Fermentat Ind, Shanghai 200237, Peoples R China;[2]Shanghai Changzheng Hosp, Organ Transplant Ctr, Shanghai 200003, Peoples R China;[3]Shanghai Jiao Tong Univ, Shanghai Canc Inst, Renji Hosp, State Key Lab Oncogenes & Related Genes,Sch Med, Shanghai 200032, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol SC, Shanghai 200237, Peoples R China

年份：2020

卷号：162

起止页码：262

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20242616313825);WOS:【SCI-EXPANDED(收录号:WOS:000577936100025)】；

基金：This work was financially supported by National Key Research & Development Program of China (2019YFD0901805, 2017YFB0309302).

语种：英文

外文关键词：Chitooligosaccharides; Poly(lactic-co-glycolic acid); Nanoparticles

摘要：The nano drug delivery system (NDDS) has been extensively investigated for cancer treatment because of its ability to enhance drug efficacy. However, there are only a few studies attempting NDDS for AZD9291 (Osimertinib). Here, we encapsulated AZD9291 in chitooligosaccharides (COS)-modified poly (lactic -co-glycolic acid) (PLGA) nanoparticles. COS, a cationic polymer, was used to develop positively charged nanoparticles with good biological affinity. The prepared AZD-PLGA-COS NPs exhibited a smaller particle size (176.6 0.4 nm), a positively charged surface (+18.65 +/- 0.38 mV), and an increased cellular uptake. The IC50 of H1975 cells was reduced by 45.90%, and the expression of p-EGFR, PARP, Bak, caspase-9, Bax, and Bcl-2 was regulated to promote cellular apoptosis. Furthermore, COS was found to inhibit the expression of immune checkpoint PD-L1. This study suggests that COS-modified PLGA nanoparticles with low toxicity and high encapsulation efficiency (EE) could potentially enhance drug efficacy. (c) 2020 Published by Elsevier B.V.