文献类型：期刊文献

英文题名：Metabolism profile of scutellarin in urine following oral administration to rats by ultra performance liquid chromatography coupled to time-of-flight mass spectrometry

作者：Liu, Qingfei[1];Shi, Yun[2];Wang, Yong[3];Lu, Juan[1];Cong, Wenjuan[3];Luo, Guoan[1];Wang, Yiming[1]

机构：[1]Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China;[2]Guangzhou Univ Tradit Chinese Med, Hosp 1, Guangzhou 510405, Guangdong, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2009

卷号：80

期号：1

起止页码：84

外文期刊名：TALANTA

收录：;EI(收录号：20094012353900);WOS:【SCI-EXPANDED(收录号:WOS:000271055700011)】；

基金：The project was supported by the National Natural Science Fund (No. 30500666) and the Tsinghua Yuyuan Medical Fund (No. 20240000548).

语种：英文

外文关键词：UPLC/TOF MS; Scutellarin; Metabolism profile; Rat; Urine

摘要：Scutellarin, a flavone glucuronide of 5,6,4'-trihydroxyflavone-7-O-glucoronide, is the main active component of the traditional Chinese botanic drug Erigeron breviscapus (Vant.) Hand.-Mazz. in this study, a method based on ultra performance liquid chromatography coupled with a time-of-flight mass spectrometer (UPLC/TCF MS) was established and validated to profile the metabolites of scutellarin in Sprague-Dawley rat urine following oral administration of single dose of scutellarin at 80.8 mg/kg. The column utilized was an Acquity BEH C18 (150 mm x 2.1 mm, 1.7 mu m). The mobile phase was 0.2% formic acid and acetonitrile with gradient condition. Two standard curves of scutellarin were obtained for the concentration range of 1.065-10.65 mu g/ml. and 10.65-63.92 mu g/ml., respectively. By automating the data processing of the software Masslynx developed by Waters Ltd., 17 metabolites of scutellarin were found and determined in rat urine, with the corresponding reactions in vivo such as isomerism, reduction, methylation, glucuronide conjugation, hydroxylation, hydroxylation and methylation, etc., most of which were discovered for the first time. For most metabolites, the time (T-p) of peak excretion was 8-12 h. Calculated as scutellarin, the cumulative urine excretion rate of the metabolites was 1.93%. (C) 2009 Elsevier B.V. All rights reserved.